Results 81 to 90 of about 246,066 (298)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Framing Huawei: from the target of national debate to the site of international conflict in Canada’s mainstream online newspaper

Online Media and Global Communication
This study was conducted to investigate the Canadian mainstream online media report of Huawei, the Chinese IT company and critical actor in China’s OFDI attempt, and how the media deviate or conform to the Government’s and the social and political elites’
Deng Yiheng, Xu Yang, Koch Pamela
doaj   +1 more source

Debating Employment in National TV News: Depoliticised Discourses and Overlooked EU Policies

Politics and Governance
This article examines the framing of employment policies in public debates within European Union (EU) member states. (Mediatised) public debate is not merely a medium for discussing employment policy; it constitutes a normative infrastructure of ...
Damien Pennetreau
doaj   +1 more source

Amplification of Thermostable Neutral Protease Open Reading Frame from Geobacillus sp. DS3 Isolated from Sikidang Crater, Dieng Plateau, Central Java, Indonesia [PDF]

, 2022
Lucia Dhiantika Witasari, Joshua Halim, Caroline Jilbert, Muhammad Saifur Rohman   +3 more
openalex   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Using Animal Welfare Framing as an Effective Approach to Communicating Climate Change—A Review

Veterinary Medicine and Science
Introduction Climate change and its effects continue to threaten the stability of environments worldwide, impacting nearly every species. Although framing is an established technique in climate communication science, little has been done in communicating
Collin Kramer, Jessica R. Page, Jaylene Flint, Mark Flint   +3 more
doaj   +1 more source

Analisis Framing cnnindonesia.com Dan detik.com Dalam Pemberitaan Konflik Palestina-Israel Pasca Tewasnya Ismail Haniyeh

Jurnal Keislaman
The conflict between Palestine and Israel, triggered by Hamas' attack on Israel on October 7, 2023, is still ongoing. Although there were some mediation and negotiations, there was no agreement between Palestine and Israel.
Sinta Dwi Utami, Abdul Azis, Iswahyu Pranawukir   +2 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source