Results 121 to 130 of about 1,439,807 (264)

Serum-free culture with PVP enables maintenance of primary culture of parathyroid cells in vitro. [PDF]

Biosci Rep
Iwawaki N, Takehara T, Tatemizo A, Teramura T.   +3 more
europepmc   +1 more source

Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells. [PDF]

Cell Transplant, 2023
Lin CY, Ching YY, Wu SF, Lee YK, Fan HC, Su LY, Tsai SY, Chen YC, Shen CI, Su HL.   +9 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Cytoprotective Effects of Human Platelet Lysate during the Xeno-Free Culture of Human Donor Corneas. [PDF]

Int J Mol Sci, 2023
Talpan D, Salla S, Meusel L, Walter P, Kuo CC, Franzen J, Fuest M.   +6 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Animal-origin-free culture improves mitotic response for chromosome aberration analysis of human peripheral blood. [PDF]

PNAS Nexus
Fujishima Y, Anderson D, Kamakura S, Saito R, Tsushima Y, Okimoto A, Goh VST, Miura T.   +7 more
europepmc   +1 more source

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering. [PDF]

Stem Cell Res Ther, 2023
Parvin Nejad S, Lecce M, Mirani B, Machado Siqueira N, Mirzaei Z, Santerre JP, Davies JE, Simmons CA.   +7 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Transition to fetal calf serum-free culture enhances kidney proximal tubule cell bioenergetics and allows pharmacological applications. [PDF]

Cell Tissue Res
van der Made TK, Cleijpool P, Paul P, Barnes DA, Oswald S, Broeksma BC, Faucher Q, Mihăilă SM, Masereeuw R.   +8 more
europepmc   +1 more source

Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum- and protein-free culture medium. [PDF]

Anat Histol Embryol, 2022
Himelreich Perić M, Mužić-Radović V, Marić T, Bulić-Jakuš F, Jurić-Lekić G, Takahashi M, Sinčić N, Ježek D, Katušić-Bojanac A.   +8 more
europepmc   +1 more source