Results 151 to 160 of about 1,439,807 (264)

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Outcomes from an intervention study implementing fee-free culture and susceptibility urine testing in dogs and cats. [PDF]

Vet Rec
Hii AW, Gilkerson JR, Bailey KE, Scarborough R, Sri AE, Awad M, Etheridge L, Hardefeldt LY.   +7 more
europepmc   +1 more source

Different transcriptional profiles of human embryonic stem cells grown in a feeder-free culture system and on human foreskin fibroblast feeder layers. [PDF]

Aging (Albany NY), 2022
Xiao L, Zhu J, Liu Z, Wu B, Zhou X, Wei Y, Sun F, Wang Z, Quan S, Li Q, Wang J, Huang L, Ma Y.   +12 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Evaluating the Influence of Different Serum-Free Culture Conditions on the Production and Function of Natural Killer Cell-Derived Extracellular Vesicles. [PDF]

J Extracell Biol
Wu Y, Chollet H, Sudworth A, Inngjerdingen M.   +3 more
europepmc   +1 more source

Serum free culture for the expansion and study of type 2 innate lymphoid cells. [PDF]

Sci Rep, 2021
de Lucía Finkel P, Sherwood C, Saranchova I, Xia W, Munro L, Pfeifer CG, Piret JM, Jefferies WA.   +7 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Development of Serum-Free Culture Systems for an Immortalized Porcine Kidney-Derived Macrophage Cell Line. [PDF]

Animals (Basel)
Haraguchi S, Takenouchi T, Masujin K, Suzuki S, Kokuho T, Uenishi H.   +5 more
europepmc   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

In vitro differentiation of common lymphoid progenitor cells into B cell using stromal cell free culture system. [PDF]

BMC Immunol
Yang P, Chen X, Wen H, Yu M, Yu H, Wang L, Gong L, Zhao L.   +7 more
europepmc   +1 more source