Results 81 to 90 of about 7,311,766 (303)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

An optically powered, free space optical communications receiver

2008 IEEE International Symposium on Circuits and Systems (ISCAS), 2008
Two of the key components of sensor motes are a power source and a communications receiver. One approach to creating very small motes is to use free space optical communication and to scavenge power from the optical downlink. In this paper photodiodes are described that were made on a standard CMOS process and generate electrical power from the ...
Liu, J, Faulkner, G, Collins, S, O'Brien, D, Elston, S   +4 more
openaire   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Diffractive optical computing in free space

Nature Communications
AbstractStructured optical materials create new computing paradigms using photons, with transformative impact on various fields, including machine learning, computer vision, imaging, telecommunications, and sensing. This Perspective sheds light on the potential of free-space optical systems based on engineered surfaces for advancing optical computing ...
Jingtian Hu, Deniz Mengu, Dimitrios C. Tzarouchis, Brian Edwards, Nader Engheta, Aydogan Ozcan   +5 more
openaire   +3 more sources

Lambertian source modelling of free space optical ground-to-train communications [PDF]

, 2012
There is a increasing demand for high speed data services while travelling on trains and other means of public transportation. Hence, an alternative technology to radio frequency (RF) communications called free space optics (FSO) could be used to provide
Ghassemlooy, Zabih, H. Le Minh, Paudel, Rupak, R. Paudel, Z. Ghassemlooy, E. Leitgeb, Leitgeb, Erich, Rajbhandari, Sujan, S. Rajbhandari, Le Minh, Hoa   +9 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

The effect of atmospheric turbulence on the performance of the free space optical communications [PDF]

, 2008
In free space optical (FSO) communication links, atmospheric turbulence has a significant impact on the quality of a laser beam propagating through the atmosphere.
Ghassemlooy, Zabih, A. Chaman Motlagh, Z. Ghassemlooy, Ahmadi, Vahid, Chaman Motlagh, Abolfazl, V. Ahmadi, Abedi, Kambiz, K. Abedi   +7 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source