Results 61 to 70 of about 446,336 (299)
Reprogramming tumor cells with LCOR mRNA nanotherapy to enhance immunotherapy
Molecular Oncology, EarlyView.LCOR (Ligand‐dependent corepressor) mRNA encapsulated in polymeric nanoparticles restores LCOR activity in tumor cells, constraining aggressive tumor cell states and increasing tumor immunogenicity. This reprogramming makes tumor cells more vulnerable to T‐cell attack and enhances their response to immune‐checkpoint immunotherapy.
Gabriel Serra‐Mir +9 more
wiley +1 more source
Advanced control in freeze-drying
, 2018 The concept of modern closed-loop control was introduced for freeze-dryers in the pharmaceutical field in the early Sixties. Among various drying processes, freeze-drying stands out as the liquid, usually water, is removed at low temperature; this allows
Barresi A. A. +5 more
core +1 more source
Molecular Oncology, EarlyView.This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan +8 more
wiley +1 more source
Development of novel containment systems for freeze-drying
, 2013 This thesis investigates the novel use of paper and Tyvek sterilisation pouches as containment systems to perform various applications of freeze-drying.
Christopher Cherry (4434256)
core +1 more source
The Indian Journal of Agricultural Sciences, 2015 Aloe (Aloe barbadensis Mill.) is commercially cultivated for its transparent leaf gel and leaf exudates. The leaf exudates collected from epidermal layer contain anthraquinone glycosides (aloins) mainly barbaloin (aloin A) and isobarbaloin (aloin B ...
RAJU SARAVANAN +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function
FEBS Open Bio, EarlyView.Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
A new concept for the continuous freeze-drying of pharmaceutical products in unit doses [PDF]
, 2018 The feasibility of the continuous freeze-drying technology has been studied simulating the process using a functional version of the continuous freeze-dryer.
Roberto Pisano
core
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source