Results 201 to 210 of about 85,630 (266)
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Journal of Microscopy, 1974
SUMMARYFreeze‐drying is a useful way to maintain the three‐dimensional morphology of biological materials for electron microscopy. The process is achieved simply by utilizing a commercially available thermoelectric unit within a standard vacuum evaporator.
I J, Bendet, N I, Rizk
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SUMMARYFreeze‐drying is a useful way to maintain the three‐dimensional morphology of biological materials for electron microscopy. The process is achieved simply by utilizing a commercially available thermoelectric unit within a standard vacuum evaporator.
I J, Bendet, N I, Rizk
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2014
Freeze-drying has become one of the most important processes for the preservation of biological products. This chapter provides protocols for freeze-drying of proteins and discusses the importance of formulation, cycle development, and validation. Specific formulations for stabilization of proteins are presented as well as advice on common problems ...
Baolin, Liu, Xinli, Zhou
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Freeze-drying has become one of the most important processes for the preservation of biological products. This chapter provides protocols for freeze-drying of proteins and discusses the importance of formulation, cycle development, and validation. Specific formulations for stabilization of proteins are presented as well as advice on common problems ...
Baolin, Liu, Xinli, Zhou
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Proceedings of the Royal Society of London. Series B. Biological Sciences, 1975
Abstract Most biological substances are unstable during storage owing to their high water content. This is why numerous attempts have been made over the last 100 years to prevent, by low temperature freezing, metabolic and biochemical degradations.
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Abstract Most biological substances are unstable during storage owing to their high water content. This is why numerous attempts have been made over the last 100 years to prevent, by low temperature freezing, metabolic and biochemical degradations.
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Freeze-Substitution and Freeze-Drying
1987Freeze-substitution (FS) and freeze-drying (FD) are dehydration techniques by which the water is gently removed from a frozen specimen. Both techniques can serve as a link between cryofixation and conventional thin sectioning at room temperature (Fig. 1).
Steinbrecht, R., Müller, M.
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Freezing and freeze-drying of microorganisms
Cryobiology, 1964Some papers reporting the effects of intense cold on microorganisms had already appeared in the 19th century and since then there have been a great many investigations of the freezing of microorganisms.3* 53 ‘I, 12 These experiments demonstrate that a wide variety of microorganisms can survive cooling to and storage at very low temperatures.
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2016
Freezing of biological materials is commonly carried out to delay biochemical and chemical changes resulting in loss of activity. However, the physicochemical background of freezing is not well understood. Freeze-drying is generally aimed at preserving biological materials and bioactive components for long periods of time without the need for frozen ...
Yrjö H. Roos +2 more
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Freezing of biological materials is commonly carried out to delay biochemical and chemical changes resulting in loss of activity. However, the physicochemical background of freezing is not well understood. Freeze-drying is generally aimed at preserving biological materials and bioactive components for long periods of time without the need for frozen ...
Yrjö H. Roos +2 more
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The Principles of Freeze-Drying
2014This chapter provides an up-to-date overview of freeze-drying (lyophilization) with particular relevance to stabilizing live cells or viruses for industrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements ...
Gerald D J, Adams +2 more
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2002
Given the increasing importance of reducing development time for new pharmaceutical products, formulation and process development scientists must continually look for ways to "work smarter, not harder." Within the product development arena, this means reducing the amount of trial and error empiricism in arriving at a formulation and identification of ...
Steven L, Nail +3 more
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Given the increasing importance of reducing development time for new pharmaceutical products, formulation and process development scientists must continually look for ways to "work smarter, not harder." Within the product development arena, this means reducing the amount of trial and error empiricism in arriving at a formulation and identification of ...
Steven L, Nail +3 more
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Freezing and Freeze-Drying of Bull Spermatozoa
American Journal of Physiology-Legacy Content, 1957The possible dependance of survival upon the time spermatozoa are in contact with the protective substance glycerol, prior to exposure to low temperatures, was investigated. Pretreatment for 5 minutes provided at least as much protection as 18 hours.
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The Principles of Freeze-Drying
2007This chapter provides an up-to-date overview of freeze-drying (lyophilization) with particulars relevance to stabilizing live cells or viruses for industrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements ...
openaire +2 more sources