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Freeze-etching nomenclature

Science, 1975
N B Gilula   +2 more
exaly   +3 more sources

Freeze Fracture and Freeze Etching

2013
Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior. These large panoramas reveal the three-dimensional contours of membranes making the methods well suited to studying changes in membrane architecture.
Douglas E, Chandler, William P, Sharp
openaire   +2 more sources

Complementary freeze‐fracture, freeze‐etch specimens

Journal of Microscopy, 1979
SUMMARYA procedure is described for the preparation and comparison of complementary freeze‐fracture‐freeze‐etch specimens. These complementary replicas reveal the value of etching some specimens even when the cryoprotectant concentration amounts to as much as 25% glycerol and 25% sucrose.
R L, Steere, E F, Erbe
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Freeze-Etching of Bacteria

1972
Publisher Summary The first electron micrographs of bacterial cells were published about 20 years ago. These early studies suggested the presence of definite intracellular structures. Sectioning techniques, originally developed for eucaryotic cells, were gradually modified so that thin sections of bacteria could be obtained. These first thin sections
C C, Remsen, S W, Watson
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Visualization of ribosomes by freeze-etching

Experimental Cell Research, 1970
Abstract Ribosomes are revealed on the outer surface of the nucler envelope by etching freeze-fractured nuclei isolated from sea urchin embryos. Etching techniques provide a novel way of investigating ribosome-membrane association.
J, Wartiovaara, D, Branton
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Freeze-etching studies on muscle

Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971
Abstract The technique of freeze-etching is illustrated with reference to striated muscle. Besides features of immediate biological interest, the material demonstrates various ways in which the process may be used in general to yield new information.
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Freeze-Etching and Freeze-Fracturing

1973
The freeze-etching (or fracturing) technique involves the making of a platinum-carbon replica of the fracture face through frozen cells. This replica is examined in the electron microscope. There are two advantages compared with earlier techniques. Firstly, fixation and dehydration are not necessary and the replica is thus of cells which differ from ...
openaire   +1 more source

Freeze‐etching and dental research

Journal of Periodontal Research, 1972
Dental plaque is the aetiologie factor common to caries and chronic periodontitis. Understanding of the structure of this microbial film has been hindered by the fact that conventional methods of fixation and embedding are more or less disruptive in their effects.
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Some artifacts of the freeze-etching technique

Journal of Ultrastructure Research, 1972
Polystyrene latex spheres 88 nm in diameter were mixed with a range of concentrations of glycerol from 0% to 25 % and used in a model system to investigate some artifacts of the fracturing and etching stages of the freeze-etching technique. In every case stretching artifacts of the latex were observed in the fracture plane, and there was a tendency for
W F, Dunlop, A W, Robards
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Freeze-etched surfaces in potato starch

Journal of Ultrastructure Research, 1972
Native and Lintnerized potato starch grains were freeze-fractured and freezeetched. All samples showed a granular surface. The Lintnerized samples, in addition, presented unique structures which have been described as microfibrils. The question, whether the granules and the microfibrils seen here are real structures in the starch grain or artifacts ...
R, Leonard, C, Sterling
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