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New Dimensions in Freeze-Etching
Proceedings, annual meeting, Electron Microscopy Society of America, 1969A redesigned specimen holder and cap have made possible the freeze-etching of both fracture surfaces of a frozen fractured specimen. In principal, the procedure involves freezing a specimen between two specimen holders (as shown in A, Fig. 1, and the left side of Fig. 2).
Russell L. Steere, Michael Moseley
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Freeze-etching studies of membrane structure
Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971Thin sections of biological membranes examined by electron microscopy appear as two dark lines separated by a lighter space. This observation, first made in the 1950s, has been interpreted as confirmation of the Danielli-Davson model of the biological membrane (Robertson 1959) .
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Freeze-Etching of Malarial Parasites
Proceedings, annual meeting, Electron Microscopy Society of America, 1969The freeze-etching technique introduced by Steere permits the rapid preparation of replicas of biological specimens for ultrastructural study without subjecting the specimens to drastic procedures of long fixation, dehydration and embedding. Fresh cells or cells briefly exposed to glutaraldehyde are frozen in 40% glycerol at -190°C and fractured under ...
Roger L. Ladda, Russell L. Steere
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Preparing Hydra for Freeze-Fracture and Freeze-Etching
1983The freeze-fracture technique is particularly advantageous for the study of cell surfaces and intercellular junctions. In general, it involves the rapid freezing of tissues, either with or without partial sublimation of ice (etching). By evaporating a film of pure carbon on the exposed surface, a replica of the cell surface is obtained which reveals ...
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Journal of Neuropathology and Experimental Neurology, 1980
FRANÇOIS X. OMLIN +2 more
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FRANÇOIS X. OMLIN +2 more
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FREEZE ETCHING: A CLASSICAL VIEW*
Annals of the New York Academy of Sciences, 1972openaire +3 more sources
On freeze-fracture and freeze-etch instrumentation
Ultramicroscopy, 1979J. Vos, I. Stokroos, G. Kors
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