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Freeze-Etching in Neuroanatomy

1981
The application of the freeze-etching technique as described by Moor and Muhlethaler (1963) to neural tissue was introduced by Bischoff (Bischoff and Moor 1967 a, b) for the study of myelin and by Akert et al. (1969) in an attempt to visualize the active sites of the presynaptic membrane.
K. Akert, C. Sandri, H. Moor
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Flagellar necklaces: Freeze-etch observations

Journal of Ultrastructure Research, 1973
Through use of the freeze-etch technique, the presence of a 3-stranded particulate necklace is demonstrated for spermatozoa of the earthworm. It is located in the region of the flagellum adjacent to the mitochondria; the component particles are ca. 100 A in diameter, and the 3 strands are spaced ca. 250–300 A from one another.
B H, Bergstrom, C, Henley
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Complementary freeze-fracture and freeze-etch preparations

Proceedings, annual meeting, Electron Microscopy Society of America, 1984
Whereas freeze-etching technology was introduced in 1957 (1) and the first freeze-etching equipment to be made available commercially was developed in 1961 (2), the need for complementary replicas was not recognized until Branton (3) proposed that the two membrane faces seen in freeze-etched specimens were not the inner and outer surfaces of membranes ...
Russell L. Steere   +2 more
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Freeze‐fracturing and freeze‐etching of cardiac myosin filaments

Journal of Microscopy, 1975
SUMMARYMyofilament structure was studied in freeze‐etch replicas of unfixed, glycerinated beef cardiac muscle. The information which is revealed depends upon the direction of metal shadowing in relation to the filament axis. Shadows oblique to this axis reveal that the outer surface of a longitudinal half of a thick filament comprises three, sometimes ...
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Freeze-etch study of the teleostean pituitary

Cell and Tissue Research, 1979
The structure of membranes in the pituitary of several teleostean species was studied with the freeze-etching technique. Nuclear pore density is higher in hormone-secreting than in stellate cells, suggesting a higher rate of nucleo-cytoplasmic interactions in the secretory cells. The perinuclear endoplasmic reticulum (ER) in the MAS-secreting cells has
M, Abraham, C, Sandri, K, Akert
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Freeze-etching studies of membrane structure

Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971
Abstract Thin sections of biological membranes examined by electron microscopy appear as two dark lines separated by a lighter space. This observation, first made in the 1950s, has been interpreted as confirmation of the Danielli-Davson model of the biological membrane (Robertson 1959) .
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Studies on Freeze-Etching of Cell Membranes

1971
Publisher Summary Freeze-etching permits the study of four different views of a membrane. When isolated membrane vesicles are frozen and the ice subsequently removed by sublimation, the real outer or inner surface is disclosed. These surfaces are covered by a number of particles attached to a smooth layer.
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ULTRASTRUCTURE OF THE NEUROHYPOPHYSIS AS SHOWN BY FREEZE-ETCHING

Journal of Endocrinology, 1970
SUMMARY Freeze-etched preparations of rabbit neurohypophysis and isolated hormone granules from cow neurohypophyses were examined electron microscopically. Many features seen in conventionally prepared sections were also apparent in freeze-etched replicas, particularly the neurosecretory nerve-endings containing elementary granules ...
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Limitations and Prospects of Freeze-Fixation and Freeze-Etching

Proceedings, annual meeting, Electron Microscopy Society of America, 1977
Ever since the freeze-etching method became a routine laboratory technique, an increasing number of scientists has applied it in biological and especially in membrane research. The manifold details of membrane architecture gave rise to a multitude of questions as to the functional significance of these structures, the possible introduction of ...
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FREEZE ETCHING: A CLASSICAL VIEW*

Annals of the New York Academy of Sciences, 1972
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