Results 21 to 30 of about 2,676 (110)

Simultaneous quantitative live cell imaging of multiple FRET-based biosensors. [PDF]

open access: yesPLoS ONE, 2013
We have developed a novel method for multi-color spectral FRET analysis which is used to study a system of three independent FRET-based molecular sensors composed of the combinations of only three fluorescent proteins.
Andrew Woehler
doaj   +1 more source

FRET-Based Ca2+ Biosensor Single Cell Imaging Interrogated by High-Frequency Ultrasound

open access: yesSensors, 2020
Fluorescence resonance energy transfer (FRET)-based biosensors have advanced live cell imaging by dynamically visualizing molecular events with high temporal resolution.
Sangpil Yoon   +3 more
doaj   +1 more source

Anomalous surplus energy transfer observed with multiple FRET acceptors. [PDF]

open access: yesPLoS ONE, 2009
BACKGROUND:Förster resonance energy transfer (FRET) is a mechanism where energy is transferred from an excited donor fluorophore to adjacent chromophores via non-radiative dipole-dipole interactions.
Srinagesh V Koushik   +2 more
doaj   +1 more source

Understanding of Förster Resonance Energy Transfer (FRET) in Ionic Materials

open access: yesSustainable Chemistry, 2021
Herein, an ionic material (IM) with Förster Resonance Energy Transfer (FRET) characteristics is reported for the first time. The IM is designed by pairing a Nile Blue A cation (NBA+) with an anionic near-infrared (NIR) dye, IR820−, using a facile ion ...
Amanda Jalihal   +6 more
doaj   +1 more source

Multiprobe NSOM fluorescence

open access: yesNanophotonics, 2014
In this paper, we demonstrate simultaneous AFM/NSOM using a dual-tip normal tuning-fork based scanning probe microscope. By scanning two SPM probes simultaneously, one dedicated for AFM with a standard tip diameter of 20 nm, and the second having a 150 ...
Berezin Shirly   +4 more
doaj   +1 more source

Three-Dimensional Reconstruction of Three-Way FRET Microscopy Improves Imaging of Multiple Protein-Protein Interactions. [PDF]

open access: yesPLoS ONE, 2016
Fluorescence resonance energy transfer (FRET) microscopy is a powerful tool for imaging the interactions between fluorescently tagged proteins in two-dimensions. For FRET microscopy to reach its full potential, it must be able to image more than one pair
Brandon L Scott, Adam D Hoppe
doaj   +1 more source

Oxidation-Responsive Micelles for Drug Release Monitoring and Bioimaging of Inflammation Based on FRET Effect in vitro and in vivo

open access: yesInternational Journal of Nanomedicine, 2022
Ziqiang Zhou,1 Yanfang Wang,2 Ping Hu1 1College of Pharmacy, Jinan University, Guangzhou, People’s Republic of China; 2First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, People’s Republic of ChinaCorrespondence: Ping Hu ...
Zhou Z, Wang Y, Hu P
doaj  

Off-Resonance Control and All-Optical Switching: Expanded Dimensions in Nonlinear Optics

open access: yesApplied Sciences, 2019
The theory of non-resonant optical processes with intrinsic optical nonlinearity, such as harmonic generation, has been widely understood since the advent of the laser.
David S. Bradshaw   +2 more
doaj   +1 more source

Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen. [PDF]

open access: yesPLoS ONE, 2020
Förster Resonance Energy Transfer (FRET) has become an immensely powerful tool to profile intra- and inter-molecular interactions. Through fusion of genetically encoded fluorescent proteins (FPs) researchers have been able to detect protein ...
Tyler W McCullock   +2 more
doaj   +1 more source

Adventures in FRET and Specification [PDF]

open access: yes
This paper gives an overview of previous work in which the authors used NASA's Formal Requirement Elicitation Tool (FRET) to formalise requirements. We discuss four case studies where we used FRET to capture the system's requirements. These formalised requirements subsequently guided the case study specifications in a combination of formal paradigms ...
Marie Farrell   +4 more
openaire   +3 more sources

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