Results 21 to 30 of about 58,345 (258)
Development of a Fluorescent Protein Based FRET Biosensor for Determination of Protease Activity
Proteases are closely associated with many pathological conditions. Efficient detection of protease activity may be useful for diagnosis, prognosis, and the development of new therapeutic biomolecules.
İsa Gökçe +3 more
doaj +1 more source
FRET-Based Ca2+ Biosensor Single Cell Imaging Interrogated by High-Frequency Ultrasound
Fluorescence resonance energy transfer (FRET)-based biosensors have advanced live cell imaging by dynamically visualizing molecular events with high temporal resolution.
Sangpil Yoon +3 more
doaj +1 more source
Anomalous surplus energy transfer observed with multiple FRET acceptors. [PDF]
BACKGROUND:Förster resonance energy transfer (FRET) is a mechanism where energy is transferred from an excited donor fluorophore to adjacent chromophores via non-radiative dipole-dipole interactions.
Srinagesh V Koushik +2 more
doaj +1 more source
Understanding of Förster Resonance Energy Transfer (FRET) in Ionic Materials
Herein, an ionic material (IM) with Förster Resonance Energy Transfer (FRET) characteristics is reported for the first time. The IM is designed by pairing a Nile Blue A cation (NBA+) with an anionic near-infrared (NIR) dye, IR820−, using a facile ion ...
Amanda Jalihal +6 more
doaj +1 more source
Three-Dimensional Reconstruction of Three-Way FRET Microscopy Improves Imaging of Multiple Protein-Protein Interactions. [PDF]
Fluorescence resonance energy transfer (FRET) microscopy is a powerful tool for imaging the interactions between fluorescently tagged proteins in two-dimensions. For FRET microscopy to reach its full potential, it must be able to image more than one pair
Brandon L Scott, Adam D Hoppe
doaj +1 more source
Ziqiang Zhou,1 Yanfang Wang,2 Ping Hu1 1College of Pharmacy, Jinan University, Guangzhou, People’s Republic of China; 2First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, People’s Republic of ChinaCorrespondence: Ping Hu ...
Zhou Z, Wang Y, Hu P
doaj
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
In this paper, we demonstrate simultaneous AFM/NSOM using a dual-tip normal tuning-fork based scanning probe microscope. By scanning two SPM probes simultaneously, one dedicated for AFM with a standard tip diameter of 20 nm, and the second having a 150 ...
Berezin Shirly +4 more
doaj +1 more source
Off-Resonance Control and All-Optical Switching: Expanded Dimensions in Nonlinear Optics
The theory of non-resonant optical processes with intrinsic optical nonlinearity, such as harmonic generation, has been widely understood since the advent of the laser.
David S. Bradshaw +2 more
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source

