Results 21 to 30 of about 58,345 (258)

Development of a Fluorescent Protein Based FRET Biosensor for Determination of Protease Activity

open access: yesSakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2021
Proteases are closely associated with many pathological conditions. Efficient detection of protease activity may be useful for diagnosis, prognosis, and the development of new therapeutic biomolecules.
İsa Gökçe   +3 more
doaj   +1 more source

FRET-Based Ca2+ Biosensor Single Cell Imaging Interrogated by High-Frequency Ultrasound

open access: yesSensors, 2020
Fluorescence resonance energy transfer (FRET)-based biosensors have advanced live cell imaging by dynamically visualizing molecular events with high temporal resolution.
Sangpil Yoon   +3 more
doaj   +1 more source

Anomalous surplus energy transfer observed with multiple FRET acceptors. [PDF]

open access: yesPLoS ONE, 2009
BACKGROUND:Förster resonance energy transfer (FRET) is a mechanism where energy is transferred from an excited donor fluorophore to adjacent chromophores via non-radiative dipole-dipole interactions.
Srinagesh V Koushik   +2 more
doaj   +1 more source

Understanding of Förster Resonance Energy Transfer (FRET) in Ionic Materials

open access: yesSustainable Chemistry, 2021
Herein, an ionic material (IM) with Förster Resonance Energy Transfer (FRET) characteristics is reported for the first time. The IM is designed by pairing a Nile Blue A cation (NBA+) with an anionic near-infrared (NIR) dye, IR820−, using a facile ion ...
Amanda Jalihal   +6 more
doaj   +1 more source

Three-Dimensional Reconstruction of Three-Way FRET Microscopy Improves Imaging of Multiple Protein-Protein Interactions. [PDF]

open access: yesPLoS ONE, 2016
Fluorescence resonance energy transfer (FRET) microscopy is a powerful tool for imaging the interactions between fluorescently tagged proteins in two-dimensions. For FRET microscopy to reach its full potential, it must be able to image more than one pair
Brandon L Scott, Adam D Hoppe
doaj   +1 more source

Oxidation-Responsive Micelles for Drug Release Monitoring and Bioimaging of Inflammation Based on FRET Effect in vitro and in vivo

open access: yesInternational Journal of Nanomedicine, 2022
Ziqiang Zhou,1 Yanfang Wang,2 Ping Hu1 1College of Pharmacy, Jinan University, Guangzhou, People’s Republic of China; 2First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, People’s Republic of ChinaCorrespondence: Ping Hu ...
Zhou Z, Wang Y, Hu P
doaj  

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Multiprobe NSOM fluorescence

open access: yesNanophotonics, 2014
In this paper, we demonstrate simultaneous AFM/NSOM using a dual-tip normal tuning-fork based scanning probe microscope. By scanning two SPM probes simultaneously, one dedicated for AFM with a standard tip diameter of 20 nm, and the second having a 150 ...
Berezin Shirly   +4 more
doaj   +1 more source

Off-Resonance Control and All-Optical Switching: Expanded Dimensions in Nonlinear Optics

open access: yesApplied Sciences, 2019
The theory of non-resonant optical processes with intrinsic optical nonlinearity, such as harmonic generation, has been widely understood since the advent of the laser.
David S. Bradshaw   +2 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

open access: yesFEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto   +3 more
wiley   +1 more source

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