In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET
Förster resonance energy transfer (FRET) microscopy is used in numerous biophysical and biomedical applications to monitor inter- and intramolecular interactions and conformational changes in the 2–10 nm range. FRET is currently being extended to in vivo
Jason T. Smith +5 more
doaj +1 more source
Inhibition of SARS-CoV 3CL protease by flavonoids
There were severe panics caused by Severe Acute Respiratory Syndrome (SARS) and Middle-East Respiratory Syndrome-Coronavirus. Therefore, researches targeting these viruses have been required.
Seri Jo +3 more
doaj +1 more source
What We Fret About When We Fret About Bootstrapping [PDF]
Den som färdas i staden har rätt att få svar på frågan: När kommer jag fram? Inställda turer, förseningar, trafikstockningar och brist på lediga parkeringsplatser försinkar oftast utan förvarning. Det är hög tid att förbättra tjänstens kvalité.
Blocher, Joseph
core +2 more sources
Simultaneous quantitative live cell imaging of multiple FRET-based biosensors. [PDF]
We have developed a novel method for multi-color spectral FRET analysis which is used to study a system of three independent FRET-based molecular sensors composed of the combinations of only three fluorescent proteins.
Andrew Woehler
doaj +1 more source
Development of a Fluorescent Protein Based FRET Biosensor for Determination of Protease Activity
Proteases are closely associated with many pathological conditions. Efficient detection of protease activity may be useful for diagnosis, prognosis, and the development of new therapeutic biomolecules.
İsa Gökçe +3 more
doaj +1 more source
Nanosecond Dynamics of Single-Molecule Fluorescence Resonance Energy Transfer
Motivated by recent experiments on photon statistics from individual dye pairs planted on biomolecules and coupled by fluorescence resonance energy transfer (FRET), we show here that the FRET dynamics can be modelled by Gaussian random processes with ...
Agarwal, G. S. +4 more
core +1 more source
Protein-Protein Affinity Determination by Quantitative FRET Quenching. [PDF]
The molecular dissociation constant, Kd, is a well-established parameter to quantitate the affinity of protein-protein or other molecular interactions.
Chen, Younan +7 more
core +2 more sources
FRET-Based Ca2+ Biosensor Single Cell Imaging Interrogated by High-Frequency Ultrasound
Fluorescence resonance energy transfer (FRET)-based biosensors have advanced live cell imaging by dynamically visualizing molecular events with high temporal resolution.
Sangpil Yoon +3 more
doaj +1 more source
Understanding of Förster Resonance Energy Transfer (FRET) in Ionic Materials
Herein, an ionic material (IM) with Förster Resonance Energy Transfer (FRET) characteristics is reported for the first time. The IM is designed by pairing a Nile Blue A cation (NBA+) with an anionic near-infrared (NIR) dye, IR820−, using a facile ion ...
Amanda Jalihal +6 more
doaj +1 more source
Theory of resonance energy transfer involving nanocrystals: the role of high multipoles
A theory for the fluorescence resonance energy transfer (FRET) between a pair of semiconducting nanocrystal quantum dots is developed. Two types of donor-acceptor couplings for the FRET rate are described: dipole-dipole (d-d) and the dipole-quadrupole (d-
Abramowitz M. +4 more
core +1 more source

