Results 31 to 40 of about 2,676 (110)
A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription
Cell Structure and FunctionExtracellular signal-regulated kinase (ERK) regulates multiple cellular functions through distinct activation patterns. Genetically encoded fluorescent probes are instrumental in dissecting the ERK activity dynamics in living cells.
Zhang Weisheng +4 more
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Capturing CDKs in action: Live-cell biosensors pioneer the new frontiers in cell cycle research
Cell Structure and FunctionCyclin-dependent kinases (CDKs) orchestrate cell cycle progression through precise temporal control of substrate phosphorylation. While traditional biochemical approaches and phosphoproteomics have provided valuable insights into CDK-mediated regulation,
Sachiya Nakashima +4 more
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Frontiers in Plant Science, 2013 Förster resonance energy transfer (FRET) describes excitation energy exchange between two adjacent molecules typically in distances ranging from 2 to 10 nm.
Sara Mareike Müller +4 more
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Single cell FRET analysis for the identification of optimal FRET-pairs in Bacillus subtilis using a prototype MEM-FLIM system. [PDF]
PLoS ONE, 2015 Protein-protein interactions can be studied in vitro, e.g. with bacterial or yeast two-hybrid systems or surface plasmon resonance. In contrast to in vitro techniques, in vivo studies of protein-protein interactions allow examination of spatial and ...
Ruud G J Detert Oude Weme +5 more
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Fretting about FRET: Failure of the Ideal Dipole Approximation [PDF]
Biophysical Journal, 2009 With recent growth in the use of fluorescence-detected resonance energy transfer (FRET), it is being applied to complex systems in modern and diverse ways where it is not always clear that the common approximations required for analysis are applicable.
A. MUNOZ LOSA +4 more
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Automated and optimally FRET-assisted structural modeling
Nature Communications, 2020 To overcome the limitation of FRET data being too sparse to cover all structural details, FRET experiments need to be carefully designed and complemented with simulations. Here the authors present a toolkit for automated design of FRET experiments, which
Mykola Dimura +7 more
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Scientific Reports, 2022 CRX and NRL are retina-specific transcription factors that control rod photoreceptor differentiation and synergistically activate rod phototransduction gene expression.
Xinming Zhuo, Barry E. Knox
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Fretting about FRET: Correlation between κ and R [PDF]
Biophysical Journal, 2007 Molecular dynamics simulations were used to examine the structural dynamics of two fluorescent probes attached to a typical protein, hen egg-white lysozyme (HEWL). The donor probe (D) was attached via a succinimide group, consistent with the commonly-used maleimide conjugation chemistry, and the acceptor probe (A) was bound into the protein as occurs ...
VanBeek, Darren B. +3 more
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PLoS ONE, 2015 Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The
Bobin George Abraham +5 more
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FRET Microscopy in Yeast [PDF]
Biosensors, 2019 Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells.
Michal Skruzny, Emma Pohl, Marc Abella
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