Results 121 to 130 of about 1,105,287 (296)

Homo-FRET Imaging as a tool to quantify protein and lipid clustering [PDF]

, 2010
Homo-FRET, Förster resonance energy transfer between identical fluorophores, can be conveniently measured by observing its effect on the fluorescence anisotropy.
Erik G. Hofman, Hofman, E.G., Jarno Voortman, Arjen N. Bader, Hoetzl, S., van Bergen en Henegouwen, P.M.P., Gerritsen, H.C., Bader, A.N., Gerrit van Meer, Sandra Hoetzl, Paul M. P. van Bergen en Henegouwen, van Meer, G., Hans C. Gerritsen, Voortman, J., Cell Biology, Neurobiology and Biophysics, Chemistry Research: Bijvoet Centre for Biomolecular Research, Sub Molecular Biophysics, Afd Scheikunde Algemeen, Dep Biologie, Sub Cell Biology, Biomolecular Imaging, Soft Condensed Matter and Biophysics, Membrane Enzymology   +22 more
core   +1 more source

Degradable, Skin‐Conformal Nanocomposite‐Based Bioelectronic Patches for High‐Fidelity Electrophysiology and Gesture‐Controlled Robotics

Advanced Functional Materials, EarlyView.
A degradable, skin‐conformal bioelectronic patch combines a starch‐dominated nanocomposite conductor with a gelatin substrate to enable sensitive strain sensing, high‐fidelity electrocardiography, electromyography, electrooculography, and machine‐learning‐assisted gesture control.
Ming Dong, Hongna Yuan, Shubo Liu, Abdulkadir Sanli, Emiliano Bilotti, Han Zhang, Pietro Cataldi, Karin Hing, Oliver Fenwick, Colin J. Humphreys, Dimitrios G. Papageorgiou   +10 more
wiley   +1 more source

FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility

Nature Communications, 2017
cAMP/PKA signalling plays important roles in physiology, but there are a lack of tools to spatially distinguish cAMP. Here the authors present a FRET-based cAMP biosensor they call CUTie that can directly compare cAMP signals at multiple subcellular ...
Nicoletta C. Surdo, Marco Berrera, Andreas Koschinski, Marcella Brescia, Matias R. Machado, Carolyn Carr, Peter Wright, Julia Gorelik, Stefano Morotti, Eleonora Grandi, Donald M. Bers, Sergio Pantano, Manuela Zaccolo   +12 more
doaj   +1 more source

Linear approaches to intramolecular Förster Resonance Energy Transfer probe measurements for quantitative modeling [PDF]

, 2011
Numerous unimolecular, genetically-encoded Forster Resonance Energy Transfer (FRET) probes for monitoring biochemical activities in live cells have been developed over the past decade.
Kurt I. Anderson, Kida, K., Katarzyna Kida, Birtwistle, M.R., Kriegsheim, Alexander von, Anderson, K.I., Kida Katarzyna, von Kriegsheim, A., Walter Kolch, Anderson Kurt I., Kida, Katarzyna, Schwarz, J.P., Marc R Birtwistle, Birtwistle Marc R., Birtwistle, Marc R., Alexander von Kriegsheim, Kurt I Anderson, von Kriegsheim Alexander, Juliane P Schwarz, Schwarz Juliane P., Kolch, W., Marc R. Birtwistle, Kolch Walter, Juliane P. Schwarz, et al., Birtwistle, Marc, Anderson, Kurt, Kolch, Walter, von Kriegsheim, Alexander, Schwarz, Juliane   +29 more
core   +1 more source

Managing Charge Transport and Exciton Generation for Highly Efficient, Bright, and Stable Near‐Infrared Organic Radical Light‐Emitting Diodes

Advanced Functional Materials, EarlyView.
High‐performing near‐infrared (NIR) organic light‐emitting diodes are demonstrated using an efficient and stable 800 nm‐emissive organic radical and a thermally activated delayed fluorescence (TADF) host. TADF‐assisted exciton generation combined with improved charge transport enables high external quantum efficiency (>8%), high radiance (>70,000 mW sr−
Hwan‐Hee Cho, Min‐Hsuan Chang, Yi‐Syun Chen, Tetsuro Kusamoto, Emrys W. Evans, Yuh‐Renn Wu, Richard H. Friend   +6 more
wiley   +1 more source

Advancing Human Skin Equivalents: The Crucial Role of Neurovascular Integration

Advanced Healthcare Materials, EarlyView.
This review discusses the importance of integrating vascular and peripheral nerve systems into human skin equivalents (HSEs) to better recapitulate native skin physiology. Recent advances in vascularized, innervated, and neurovascularized HSEs are highlighted, together with emerging bioengineering strategies, current challenges, and future ...
Hao Wu, Lorenzo Moroni, Nicolas Lebonvallet, Vincent Flacher, Paul Wieringa   +4 more
wiley   +1 more source

FRET imaging of Piezo1–calcium signal activation induced by chemical agonist and microfluidic shear forces

Journal of Innovative Optical Health Sciences
The discovery of Piezo channels has advanced the understanding of mechanobiology, which acts as mechanosensitive cation channels in sensing mechanical stimuli and regulating various physiological functions.
Mingxing Ouyang, Qingshun Lu, Xin Zhang, Chenyan Zhu, Teng Luo, Xiang Wang, Yingxiao Wang, Linhong Deng   +7 more
doaj   +1 more source

NeuroVisio SyncPatch: A Skin‐Conformal Multimodal EMG–Motion Sensing Platform for Quantitative Neuromuscular Rehabilitation and Performance Monitoring

Advanced Healthcare Materials, EarlyView.
NeuroVisio SyncPatch integrates skin‐conformal electromyography (EMG) electrodes with camera‐tracked markers to jointly assess muscle activity and three‐dimensional knee kinematics during rehabilitation. Parallel feedback pathways provide real‐time kinematic guidance during movement and post‐trial neuromuscular feedback.
Bohyung Choi, Yoo‐Jin Jun, Heewon Choee, Kun‐Do Lee, Heesoo Kim, Yu Seok Hwang, Choong Yeon Kim, Seongok Chae, Juhyun Lee, Minkyu Je, Jae‐Woong Jeong, Hyung‐Soon Park   +11 more
wiley   +1 more source

Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid Biosensors

Advanced Science
The RAS–RAF–MEK–ERK signaling cascade is a central component of the mitogen‐activated protein kinase (MAPK) pathway, regulating cell proliferation, differentiation, and survival, and is frequently dysregulated in cancer.
Jeong‐Min Go, Dahee Lee, Minji Kim, Kiseok Han, Gyuho Choi, ChanHui Song, Sanghyun Ahn, Yerim Lee, Jinyoung Lee, Yingxiao Wang, Jung‐Soo Suh, Hwayoung Yun, Tae‐Jin Kim   +12 more
doaj   +1 more source

Interaction of Purinergic P2X4 and P2X7 Receptor Subunits

Frontiers in Pharmacology, 2017
P2X4 and P2X7 are members of the P2X receptor family, comprising seven isoforms (P2X1–P2X7) that form homo- and heterotrimeric non-specific cation channels gated by extracellular ATP.
Markus Schneider, Kirsten Prudic, Anja Pippel, Manuela Klapperstück, Ursula Braam, Christa E. Müller, Günther Schmalzing, Fritz Markwardt   +7 more
doaj   +1 more source