Results 131 to 140 of about 1,105,287 (296)

Rational Control of Antibody Orientation for Enhancing Sensitivity and Specificity in Lateral Flow Immunoassays With Clinical Validation

open access: yesAdvanced Healthcare Materials, EarlyView.
Systematic investigation of antibody orientation strategies in lateral flow immunoassays reveals that a para‐substituted (aminomethyl)phenylboronic acid mediated probe (P‐6) combined with a biotin‐streptavidin test‐line modification achieves a synergistic 14‐ to 19‐fold sensitivity gain over conventional conjugation. Furthermore, multiplexed detection,
Pin‐Hao Wang   +5 more
wiley   +1 more source

FRET Nanoflares for Intracellular mRNA Detection: Avoiding False Positive Signals and Minimizing Effects of System Fluctuations

open access: yes, 2015
A new class of intracellular nanoprobe, termed fluorescence resonance energy transfer (FRET) nanoflares, was developed to sense mRNA in living cells. It consists of a gold nanoparticle (AuNP), recognition sequences, and flares.
He Wang (250350)   +8 more
core   +1 more source

Single‐Particle FRET Probes Heterogeneity in the Ligand Shell of Colloidal Perovskite Quantum Dots

open access: yesAdvanced Materials, EarlyView.
Single‐particle, single‐molecule Förster resonance energy transfer turns dye‐tagged ligands into nanorulers for the otherwise elusive organic shell of CsPbBr3 quantum dots. Distance variations within individual particles reveal pronounced local heterogeneity, while changes in the surrounding ligand chemistry shift the average dye–surface separation ...
Leon G. Feld   +6 more
wiley   +1 more source

Selective Laser Sintering and Graphitization for Mono‐Matrix Unibody Fabrication of Integrated Stereo‐Circuit and Stereo‐Fluidic Wearable Electronics

open access: yesAdvanced Materials, EarlyView.
A novel selective laser sintering and graphitization (SLSG) 3D printing technology enables unibody fabrication of compacted insulating structures and conductive circuits in fluidic‐integrated wearable electronics through adaptive photonic modulation.
Li Zhang, Wing Cheung Mak
wiley   +1 more source

Radiative Thermal Management for Extreme Environments: Mechanisms and Design Strategies

open access: yesAdvanced Materials, EarlyView.
In this review, we discuss the fundamental mechanisms of radiative cooling and solar heating, material and structural design strategies, hybrid approaches integrating additional heat transfer pathways, and durability‐oriented designs addressing environmental stresses.
Hyung Rae Kim   +5 more
wiley   +1 more source

FRET Imaging

open access: yes, 2003
Förster (or Fluorescence) Resonance Energy Transfer (FRET) generates fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a new photophysical framework for the phenomenon and apply it to
Jares-Erijman, E., Jovin, T.
core  

FRET-FLIM analysis shows a strong NF-YC9 by HY5 physical interaction.

open access: yes, 2016
FRET experiments were conducted in tobacco leaves through transient 35S-driven overexpression of NF-YCs tagged with mCer3 and HY5 or NF-YB2 tagged with YFP.
Jan R. Risinger (215403)   +6 more
core   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

open access: yesAdvanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang   +12 more
wiley   +1 more source

Charge‐Reversed Lipid Nanoparticles Enable Encapsulation of Pt2L4 Nanocages for In vivo Anti‐Cancer Treatment

open access: yesAdvanced Materials, EarlyView.
Charge‐reversed lipid nanoparticles (revLNPs) enable efficient encapsulation and delivery of cationic Pt2L4 nanocages. Encapsulation preserves potent anticancer activity while improving circulation, reducing vascular leakage, and promoting favorable biodistribution in vivo.
Renzo A. Knol   +16 more
wiley   +1 more source

Advances in Bio‐Integrated Edge Computing Systems for Smart and Connected Health

open access: yesAdvanced Materials, EarlyView.
This review explores advances in bio‐integrated edge computing for healthcare, focusing on co‐designing computing, sensing, and energy strategies to overcome integration and power challenges for optimized, decentralized operation. ABSTRACT Recent advances in bio‐integrated healthcare technologies have enabled continuous, high‐resolution monitoring and ...
Gyuwon Kim   +4 more
wiley   +1 more source

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