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Restriction endonuclease mapping of the frog virus 3 genome
Virology, 1983A physical map for the frog virus 3 (FV 3) genome was constructed after digestion with the following restriction endonucleases: EcoRI, HindIII, KpnI, and XbaI. Mapping of the DNA was accomplished by partial digestion and recutting, double-digestion, and Southern blot hybridization with deduction of overlaps. Although the virion DNA is physically linear,
M H, Lee, D B, Willis
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The organization of frog virus 3 as revealed by freeze-etching
Virology, 1984A variety of freeze-fracture techniques has been employed in this study with the aim of dissecting the frog virus 3 virion and obtaining further information about its architecture. The icosahedral capsid has a skew symmetry with a triangulation number of 133 or 147. The capsomers are closely packed with a center-to-center spacing of 72 A.
F, Darcy-Tripier +3 more
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Virology, 1979
Abstract A ts mutant (ts 9467) of frog virus 3 (FV3) does not synthesize late viral RNAs or proteins, although it does synthesize viral DNA. We have analyzed by polyacrylamide gel electrophoresis the RNAs and proteins produced in ts 9467-infected cells to determine the requirements for turning on late transcription. The electrophoretic pattern of the
D B, Willis, R, Goorha, A, Granoff
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Abstract A ts mutant (ts 9467) of frog virus 3 (FV3) does not synthesize late viral RNAs or proteins, although it does synthesize viral DNA. We have analyzed by polyacrylamide gel electrophoresis the RNAs and proteins produced in ts 9467-infected cells to determine the requirements for turning on late transcription. The electrophoretic pattern of the
D B, Willis, R, Goorha, A, Granoff
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The DNA-Methylase of Frog Virus 3
1990The DNA of frog virus 3 (FV3) replicates in two stages. During the first stage, genome sized DNA is synthesized in the nucleus of infected cells. This is followed by a second stage of DNA replication in the cytoplasm where concatemeric viral DNA is synthesized. FV3 DNA is methylated in the cytoplasm by a virus-coded DNA-methylase.
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Frog virus 3 DNA is heavily methylated at CpG sequences
Virology, 1980Abstract DNA extracted from frog virus 3 (FV 3) virions was resistant to the action of restriction endonucleases that cannot cleave DNA methylated at the sequence me CpG. However, viral DNA was susceptible to digestion with at least one enzyme that recognizes an identical sequence, independent of methylation.
D B, Willis, A, Granoff
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Macromolecular Synthesis in Cells Infected by Frog Virus 3
1985The best studied member of the Iridoviridae is frog virus 3 (FV3), isolated by Granoff et al. (1966) from a renal adenocarcinoma of the leopard frog, Rana pipiens. Similar isolates were made from normal frog kidney and liver (FV1, FY 2, FV5–FY24), but FV3 was chosen for further investigation because of its presence in the tumor.
D B, Willis, R, Goorha, V G, Chinchar
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1989
Frog virus 3 (FV3) is a large icosahedral virus belonging to the Iridoviridae family. The genome is double—stranded, linear DNA with a molecular weight of 100 x 106 (Kelly and Avery, 1974; Murti et al., 1982). Among animal viruses, FV3 was the first demonstrated to contain a circularly permuted and terminally redundant genome (Goorha and Murti, 1982 ...
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Frog virus 3 (FV3) is a large icosahedral virus belonging to the Iridoviridae family. The genome is double—stranded, linear DNA with a molecular weight of 100 x 106 (Kelly and Avery, 1974; Murti et al., 1982). Among animal viruses, FV3 was the first demonstrated to contain a circularly permuted and terminally redundant genome (Goorha and Murti, 1982 ...
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Virus-Cytoskeleton Interaction during Replication of Frog Virus 3
1990Frog virus 3 (FV3) induces selective and sequential changes in the eukaryotic cytoskeleton. Morphologically, the virus disrupts the microtubules, reorganizes the intermediate filaments, and disrupts and reorganizes the microfilaments. Biochemically, the virus selectively inhibits tubulin synthesis, increases the phosphorylation of vimentin, and ...
K. G. Murti, R. Goorha
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Instability of Frog Virus 3 mRNA in Productively Infected Cells
Virology, 1994Cloned DNA restriction fragments encoding representative frog virus 3 messages were used as probes to assess the stability of viral transcripts in infected fathead minnow cells. Analysis of Northern blot hybridization profiles confirmed earlier findings and revealed that in infected cells the steady-state level of representative frog virus 3 (FV3 ...
V G, Chinchar +4 more
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Transactivation of Methylated HIV-LTR by a Frog Virus 3 Protein
Virology, 1994DNA methylation has been implicated in the suppression of transcription of a large spectrum of eukaryotic genes. Frog virus 3 (FV3) contains genomic DNA that is the most extensively methylated of all known animal viruses. However, FV3 gene expression is tightly regulated in a sequential fashion in infected cells.
C M, Spangler, K, Essani
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