Results 41 to 50 of about 378,279 (252)

Simultaneous Prostate Target Biopsy Integrated With Radical Prostatectomy: A Pilot Study Omitting Preoperative Systematic Biopsy [PDF]

Journal of Urologic Oncology
Purpose This study evaluates the viability of a new method that employs transperineal targeted biopsy with frozen section analysis immediately followed by robot-assisted radical prostatectomy (RARP), bypassing the traditional systematic biopsy deemed ...
Tae Young Park, Jae Young Hwang, Seong Woo Yun, Chang Wan Hyun, Sung Goo Yoon, Seung Bin Kim, Tae Il Noh, Ji Sung Shim, Min Goo Park, Seok Ho Kang, Sung Gu Kang   +10 more
doaj   +1 more source

Establishment of a whole slide imaging-based frozen section service at a cancer center

Journal of Pathology Informatics, 2022
Background: In recent years, there has been a surge of interest in clinical digital pathology (DP). Hardware and software platforms have matured and become more affordable, and advances in artificial intelligence promise to transform the practice of ...
Sue Chang, Evita Sadimin, Keluo Yao, Stanley Hamilton, Patricia Aoun, Raju Pillai, David Muirhead, Daniel Schmolze   +7 more
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Enhancing frozen histological section images using permanent-section-guided deep learning with nuclei attention

Scientific Reports
In histological pathology, frozen sections are often used for rapid diagnosis during surgeries, as they can be produced within minutes. However, they suffer from artifacts and often lack crucial diagnostic details, particularly within the cell nuclei ...
Elad Yoshai, Gil Goldinger, Tatiana Kogan, Anna Zakharov, Miki Haifler, Natan T. Shaked   +5 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Evaluating Diagnostic Accuracy: Frozen and Permanent Sections

Journal of Nature and Science of Medicine
Context: Frozen section (FS) analysis is an intraoperative diagnostic approach that provides surgeons with timely information to guide immediate clinical decisions. Aims: The aim of this study was to evaluate the diagnostic accuracy, turnaround time (TAT)
Mohammed A. Alswayyed, Saeed O. Bahabri, Malak A. Alzahrani, Ahmad R. Almotairi, Tamadar Al Doheyan, Hisham M. Alkhalidi, Ahmed A. Alhumidi, Amany A. Fathaddin, Wajd A. Althakfi, Fadi S. Alakeel, Maria A. Arafah   +10 more
doaj   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Comparison of pre- and postoperative parathyroid hormone levels with frozen section during parathyroidectomy

The Journal of Qazvin University of Medical Sciences, 2015
Background: Due to the short half-life of parathyroid hormone (PTH), intra-operative quick PTH measurement (immediately after removal of adenoma) is beneficial to identify and evaluate the adequacy of tissue removal and to reduce reoperation and more ...
H. Parsa, M. Samimifar
doaj