Results 121 to 130 of about 56,139 (255)

Minimizing Off‐Target Effects of CRISPR‐Cas9 With Optimized sgRNA: Evaluation of Efficiency and Specificity in the Tumor Protein 53 (TP53) Region

open access: yesBiotechnology and Bioengineering, EarlyView.
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) – Cas9‐based genome editing has emerged as a widely used tool across various disciplines, ranging from molecular biology to gene therapy. This revolutionary technology, which enables precise gene editing, represents a significant advancement in biotechnology, opening new frontiers for ...
Ali Mertcan Köse   +3 more
wiley   +1 more source

Overcoming intranasal delivery barriers with ultrastable polyzwitterionic siRNA nanocages for enhanced glioblastoma therapy

open access: yesBMEMat, EarlyView.
A ROS‐responsive, zwitterionic nanocage enables stable, intranasal siRNA delivery to glioblastoma, promoting deep tumor penetration via non‐degradative pathways and trigeminal nerve transport. This platform achieves durable gene silencing and tumor suppression, offering a non‐invasive, storage‐stable strategy for treating glioma and other neurological ...
Jingwen Xie   +12 more
wiley   +1 more source

Near‐infrared light‐activated aptamer‐based nanoprobe for spatiotemporally resolved detection of L‐lactate in colorectal cancer

open access: yesBMEMat, EarlyView.
A near‐infrared (NIR) light‐activated “lock‐key” nanodevice is reported for spatiotemporally resolved detection of intratumoral L‐lactate. The system stays silent until deep‐penetrating NIR light remotely unlocks its function, allowing for precise sensing within tumors.
Siyu Pan   +8 more
wiley   +1 more source

RNA‐centric world of retroviruses: unravelling the molecular strategies of genomic RNA packaging

open access: yesBiological Reviews, EarlyView.
ABSTRACT Retroviruses constitute a unique group of RNA viruses that have profoundly influenced both evolutionary trajectories and biomedical research. Their ability to reverse transcribe and integrate into host genomes has shaped genomic architecture across species and contributed to our understanding of oncogenes, gene regulation, and RNA biology ...
Mohammad Abdullah Jehad   +5 more
wiley   +1 more source

A Modified Technique for Converting Retrograde Femoral Artery Access to Antegrade Access With the Assistance of a Long Sheath

open access: yesCatheterization and Cardiovascular Interventions, EarlyView.
ABSTRACT Background Percutaneous femoral access serves as the cornerstone of endovascular therapy of lower extremity arterial disease. In complex cases, the arterial disease of lower extremity are involve in ipsilateral iliac artery pathology, which conventional treatment strategies typically require multiple punctures or staged procedures.
Guangfeng Zheng   +6 more
wiley   +1 more source

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