Results 91 to 100 of about 557,186 (345)

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Functional annotation of variants from healthy individuals [PDF]

Genomics & Informatics, 2019
The implications of germline de novo variants (DNVs) in diseases are well documented. Despite extensive research, inconsistencies between studies remain a challenge, and the distribution and genetic characteristics of DNVs need to be precisely evaluated.
Jean Lee, Sung Eun Hong
doaj   +1 more source

Yeast Features: Identifying Significant Features Shared Among Yeast Proteins for Functional Genomics [PDF]

, 2008
Background High throughput yeast functional genomics experiments are revealing associations among tens to hundreds of genes using numerous experimental conditions.
Ashkan Golshani, Frank Dehne, James J. Cheetham, James R. Green, Md Alamgir, Michel Dumontier, Myron L. Smith, Nadereh Mir-Rashed, Veronika Eroukova   +8 more
core   +1 more source

Polymorphism identification and improved genome annotation of Brassica rapa through Deep RNA sequencing. [PDF]

, 2014
The mapping and functional analysis of quantitative traits in Brassica rapa can be greatly improved with the availability of physically positioned, gene-based genetic markers and accurate genome annotation.
Covington, Michael F, Devisetty, Upendra Kumar, Lekkala, Saradadevi, Maloof, Julin N, Tat, An V   +4 more
core   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Human gene correlation analysis (HGCA): A tool for the identification of transcriptionally co-expressed genes

BMC Research Notes, 2012
Background Bioinformatics and high-throughput technologies such as microarray studies allow the measure of the expression levels of large numbers of genes simultaneously, thus helping us to understand the molecular mechanisms of various biological ...
Michalopoulos Ioannis, Pavlopoulos Georgios A, Malatras Apostolos, Karelas Alexandros, Kostadima Myrto-Areti, Schneider Reinhard, Kossida Sophia   +6 more
doaj   +1 more source

A curated catalog of canine and equine keratin genes. [PDF]

PLoS ONE, 2017
Keratins represent a large protein family with essential structural and functional roles in epithelial cells of skin, hair follicles, and other organs. During evolution the genes encoding keratins have undergone multiple rounds of duplication and humans ...
Pierre Balmer, Anina Bauer, Shashikant Pujar, Kelly M McGarvey, Monika Welle, Arnaud Galichet, Eliane J Müller, Kim D Pruitt, Tosso Leeb, Vidhya Jagannathan   +9 more
doaj   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source