Results 211 to 220 of about 731,823 (276)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Relationship between multimorbidity, functional limitation, and quality of life among middle-aged and older adults: findings from the longitudinal analysis of the 2013-2020 Survey of Health, Ageing, and Retirement in Europe (SHARE). [PDF]

Qual Life Res
Wilk P, Ruiz-Castell M, Stranges S, Bohn T, Fagherazzi G, Nicholson K, Moran V, Makovski TT, Pi Alperin MN, Zeegers MP, Samouda H.   +10 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Weak Grip Strength and Cognition Predict Functional Limitation in Older Europeans [PDF]

Journal of the American Geriatrics Society, 2019
Objectives: To estimate the effects of baseline grip strength and cognition on the trajectory of functional limitation over time. Design: Longitudinal study of older adults participating in the Survey of Health, Ageing and Retirement in Europe (SHARE ...
Tong Wang, Yili Wu, Weilong Li
exaly   +2 more sources

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Functional Banach Limits

2021
Let \(\mathbb{R}\) denote the real line, and let \(E\) be the Banach space of bounded real-valued functions with the norm \(|f|=\text{ess sup}\{|f(x)|, x\in\mathbb{R}\}\). \(L\) denotes the set of locally integrable functions. For \(a>0\), the transform \(T_a(f)\) is defined by \[ T_a(f)(x)= (1/a) \int^{x+a}_x f(t)dt,\quad a>0,\quad T_0(f)(x)= f(x). \]
Das, G., Nanda, S.
openaire   +2 more sources