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Relevance of plant and fungal DNA metabarcoding in aerobiology

Aerobiologia, 2019
Aerobiology studies biological particles present in the air of both indoor and outdoor environments. In the past few years, the application of molecular techniques has considerably improved the knowledge of organismal diversity in air samples and highlighted its ecological and clinical importance.
Elisa Banchi, A. Pallavicini, L. Muggia
semanticscholar   +4 more sources

Fungal DNA Lysis Buffer

open access: yes, 2016
Lysis buffer for extraction of DNA from fungal material. Contains ionic and non-ionic detergents. Contains RNAse-A. Composition: NaCl 150mM EDTA pH 8.0 1 mM Tris BasepH 8.0 10 mM Polyvinylpolypyrrolidone (PVPP) 2% IGEPAL-CA630 0.5% Sodium deoxycholate 0.5% SDS 1% RNAse-A 100 µg/mL
Adam P Taranto
semanticscholar   +3 more sources

Fungal DNA extraction for Nanopore sequencing v1

, 2021
This protocol is intended for extraction of high molecular weight DNA from fungal samples.
Eri Ogiso-Tanaka   +4 more
semanticscholar   +1 more source

A simple protocol for isolation of fungal DNA

Biotechnology Letters, 2003
Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and
Sachin R. Tendulkar   +2 more
openaire   +3 more sources

Fast preparation of fungal DNA for PCR screening

Journal of Microbiological Methods, 2011
Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.
Wei-Chiang Shen   +2 more
openaire   +2 more sources

Polyamines, DNA Methylation, and Fungal Differentiation

Critical Reviews in Microbiology, 1994
Mucorales constitute a group of fungi that, because of their growth characteristics, have been used extensively in the study of cell differentiation, cell morphogenesis, and stimuli perception. We have studied the role of polyamine metabolism in the development of different Mucorales, with emphasis on Mucor and Phycomyces species.
openaire   +2 more sources

Quantitative Analysis of Fungal DNA in Chronic Rhinosinusitis

The Laryngoscope, 2004
AbstractObjectives/Hypothesis Fungi have been recognized as important pathogens in sinusitis; however, they are equally present in patients with and without sinusitis. The authors postulated that the quantity of fungal DNA in the nose is determinant of disease, is greater in patients with chronic rhinosinusitis, and is directly correlated to their ...
Andrew H. Murr   +4 more
openaire   +3 more sources

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