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Bioluminescent Immunoassay with a Protein A-Luciferase Fusion Protein
Analytical Biochemistry, 1993Protein A and firefly luciferase were genetically fused and the resulting fusion protein was applied to a bioluminescent immunoassay. The gene fusion plasmid, pMALU2, was constructed by inserting the structural gene of luciferase into a protein A expression vector, and was expressed in Escherichia coli.
T. Iwai +3 more
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2016
Albumin fusion technology can improve the blood retention properties of protein-based pharmaceutics that have a short circulation half-life. The procedure consists of fusing the HSA gene with the gene for a therapeutic protein or peptide, thus permitting the existing recombinant protein therapeutics to be reengineered using albumin as a suitable and ...
Victor Tuan Giam Chuang +2 more
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Albumin fusion technology can improve the blood retention properties of protein-based pharmaceutics that have a short circulation half-life. The procedure consists of fusing the HSA gene with the gene for a therapeutic protein or peptide, thus permitting the existing recombinant protein therapeutics to be reengineered using albumin as a suitable and ...
Victor Tuan Giam Chuang +2 more
openaire +2 more sources
Quantitative lmmunoblotting with Fusion Proteins
2003The development of vaccines against HIV-1 is currently hindered by incomplete understanding of correlates of protective immunity (1-3). Experiments are necessary to measure immune responses in sufficiently fine detail that specific protective responses can be discerned from those that are irrelevant or harmful.
Lawrence D. Loomis-Price +1 more
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Protein conjugates and fusion proteins as ocular therapeutics
Drug Discovery Today, 2019Long-acting delivery (LAD) of ocular therapeutics has potential to improve the standard of care for ocular diseases, such as age-related macular degeneration (AMD), by increasing patient compliance and reducing overall treatment burden on patients and healthcare providers.
Shrenik C. Mehta +2 more
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2018
IgG-based therapeutics has become an increasingly important category of the over two hundred biopharmaceutical products approved in the USA and the EU by late 2014. While a large percentage of this consists of monoclonal antibodies, Fc fusion proteins make up an important class of IgG-based biotechnology drugs.
Dean Pettit, Carole Heath
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IgG-based therapeutics has become an increasingly important category of the over two hundred biopharmaceutical products approved in the USA and the EU by late 2014. While a large percentage of this consists of monoclonal antibodies, Fc fusion proteins make up an important class of IgG-based biotechnology drugs.
Dean Pettit, Carole Heath
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Diphtheria Toxin Fusion Proteins
1998Two different approaches have been undertaken to develop targeted biomolecules for therapeutics. The first was the construction of immunotoxins consisting of monoclonal antibodies chemically linked through a disulfide bond to a plant or bacterial toxin or radionuclide.
James G. Krueger +4 more
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Preparation of Insoluble GST Fusion Proteins
Cold Spring Harbor Protocols, 2009INTRODUCTIONA wide variety of plasmid vectors are commercially available for the production of fusion proteins in bacterial cells. Most are also designed to incorporate a tag that allows affinity purification of the expressed fusion protein from bacterial cell extracts.
Richard G. Fehon, Ilaria Rebay
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Cytokine Fusion Protein Treatment
2002Mice suffering from melanoma can be cured by treatment with a recombinant antibody-lymphotoxin-alpha fusion protein. In order to characterize the involvement of T cells in this syngeneic tumor model, the T cell receptor repertoire was analyzed both quantitatively and qualitatively.
Jürgen C. Becker +4 more
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Antibody–cytokine fusion proteins
Archives of Biochemistry and Biophysics, 2012Cytokines are key players in stimulating and regulating immune responses in physiological and pathophysiological processes. Various cytokines have been approved for therapy of cancer and other diseases and many more are under development. However, therapeutic efficacy is often hampered by severe side effects and poor pharmacokinetic properties.
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Fusion Proteins in Retroviral Transformation
1985Publisher Summary Retroviral transforming proteins are coded for by oncogenes. The mechanism of the uptake of oncogenes by retroviruses often results in fusion with replicative genes. Fusion of oncogenes to retroviral replicative genes generates protein products that are also fused.
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