Results 121 to 130 of about 13,524,575 (343)

Impact of high-fidelity temperature feedback modeling on fusion blanket neutronics

Nuclear Fusion
Accurate modeling and simulation of neutron and gamma transport and interactions within fusion tokamaks represents a critical area of research for fusion energy development.
Jin Whan Bae, Arpan Sircar, Katarzyna Borowiec, Vittorio Badalassi, Cami Collins   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A Fusion-Spliced Near-Field Optical Fiber Probe Using Photonic Crystal Fiber for Nanoscale Thermometry Based on Fluorescence-Lifetime Measurement of Quantum Dots

Sensors, 2011
We have developed a novel nanoscale temperature-measurement method using fluorescence in the near-field called Fluorescence Near-field Optics Thermal Nanoscopy (Fluor-NOTN).
Toshiharu Saiki, Yuji Nagasaka, Yoshihiro Taguchi, Takuro Fujii   +3 more
doaj   +1 more source

Report by the Joint-Core Team for the Establishment of Technology Bases Required for the Development of a Fusion DEMO Reactor [PDF]

, 2014
In accordance with the request of the Working Group on Fusion Research, the Nuclear Science and Technology Committee, the Subdivision on R&D Planning and Evaluation, the Council for Science and Technology, the Ministry of Education, Culture, Sports ...
OKANO, Kunihiko, TANAKA, Teruya, OZAKI, Akira, TANIGAWA, Hisashi, TOBITA, Kenji, YAMADA, Hiroshi, KASADA, Ryuta, TAKENAGA, Hidenobu, SAKAMOTO, Ryuichi, USHIGUSA, Kenkichi, KANEKO, Osamu, SAKAMOTO, Yoshiteru   +11 more
core  

Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion

, 2002
Characterization of mammalian NSF (G274E) and Drosophila NSF (comatose) mutants revealed an evolutionarily conserved NSF activity distinct from ATPase-dependent SNARE disassembly that was essential for Golgi membrane fusion.
Yuval Sagiv, Zvulun Elazar, Katrin Deinhardt, Richard Newman, Graham Warren, Shima, David T., Müller, Joyce M.M., Warren, Graham, David T. Shima, James Shorter, Sagiv, Yuval, Joyce M.M. Müller, Shorter, James, Newman, Richard, Elazar, Zvulun, Deinhardt, Katrin   +15 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Approaching Semantically-Mediated Acoustic Data Fusion [PDF]

, 2007
Our primary hypothesis is that it should be possible to enrich data fusion by semantic processing, with wide potential application. In order to achieve our aim we need to represent the semantic data and enable reasoning about it in a framework that can ...
Damarla, Raju, Mark S. Nixon, Shadbolt, Nigel R, Damarla, T. Raju, Nixon, Mark S., Guo, Baofeng, Shadbolt, Nigel, T. Raju Damarla, Wang, Yi, Yi Wang, Smart, Paul, Baofeng, Guo, Nigel Shadbolt, Nixon, Mark S, Baofeng Guo, Paul Smart, Smart, Paul R, Yi, Wang   +17 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Status and issues of high-temperature and high-pressure water corrosion research of fusion structural materials in Japanese DEMO reactor development

Nuclear Fusion
The activated corrosion product assessments of fusion structural materials are essential to designing components and evaluating workers’ radiation exposure.
Motoki Nakajima, Takashi Nozawa
doaj   +1 more source