Results 161 to 170 of about 2,206,701 (242)

Decoupling Fabrication From Encoding: DNA‐Addressable Template Microparticles for Large, User‐Defined Optical Barcode Libraries

open access: yesAdvanced Science, EarlyView.
A universal hydrogel microparticle template with five DNA‐addressable domains decouples particle fabrication from barcode writing. Contact‐free multilaminar flow lithography produces >106 templates/h, and post‐fabrication domain‐selective hybridization converts one batch into large, user‐defined optical barcode libraries with robust decoding and re ...
Akihiro Eguchi   +5 more
wiley   +1 more source

Twin‐Lever Multifunctional Meta‐Optics With High Longitudinal Tolerance

open access: yesAdvanced Science, EarlyView.
We introduce a single‐layer, CMOS‐compatible metasurface that allows for polarization‐programmable, achromatic broadband dual‐mode imaging with high longitudinal tolerance. The twin‐lever design combines bright‐field focusing and vortex‐based edge‐enhanced imaging while managing axial intensity distribution from 488 to 633 nm.
Isma Javed   +10 more
wiley   +1 more source

Spatiotemporal Multi‐Omic Mapping Reveals Liver‐Muscle Metabolic Crosstalk in Cancer Cachexia

open access: yesAdvanced Science, EarlyView.
The interactive CCAtlas platform delineates cross‐species, spatiotemporal, and sex‐specific molecular dynamics and metabolic rewiring across organs during cancer cachexia. Hepatic Gamt downregulation curtails hepatic creatine synthesis to trigger systemic creatine insufficiency and consequent skeletal muscle atrophy in LLC tumour‐bearing mice ...
Zihan Tian   +12 more
wiley   +1 more source

MethyAnno: An Interpretable Automated Annotation Method Leveraging Multi‐Scale Information and Metric Learning Framework for scDNAm Data

open access: yesAdvanced Science, EarlyView.
MethyAnno enables robust and interpretable annotation of single‐cell DNA methylation data by integrating multi‐scale epigenetic information, bidirectional cross‐attention, and prototype‐based metric learning. The framework resolves rare and novel cell types across datasets while revealing cell‐type‐specific epigenetic signatures associated with disease
Yuhang Jia   +4 more
wiley   +1 more source

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

open access: yesAdvanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

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