Results 21 to 30 of about 6,069 (180)
Scalable and automated CRISPR-based strain engineering using droplet microfluidics
Microsystems & Nanoengineering, 2022 We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a chip. The microfluidic device contains a 10 × 10 element array, and each element contains sets of electrodes for two electric field ...
Kosuke Iwai +13 more
doaj +1 more source
Two siblings with galactose mutarotase deficiency: Clinical differences
JIMD Reports, 2022 Galactose mutarotase (GALM) deficiency is an inherited metabolic disease caused by the deficiency of the first enzyme in the Leloir pathway. GALM deficiency was first reported in 2018. To date, eight cases have been reported.
Havva Yazici +4 more
doaj +1 more source
The Regulatory Roles of the Galactose Permease and Kinase in the Induction Response of the GAL Network in Saccharomyces cerevisiae [PDF]
, 2006 The GAL genetic switch of Saccharomyces cerevisiae exhibits an ultrasensitive response to the inducer galactose as well as the "all-or-none" behavior characteristic of many eukaryotic regulatory networks.
Hawkins, Kristy M., Smolke, Christina D.
core +1 more source
Molecular Structure of Galactokinase [PDF]
Journal of Biological Chemistry, 2003 Galactokinase plays a key role in normal galactose metabolism by catalyzing the ATP-dependent phosphorylation of alpha-D-galactose to galactose 1-phosphate. In humans, mutations in the galactokinase gene can lead to the diseased state referred to as Type II galactosemia. Here we describe the three-dimensional structure of galactokinase from Lactococcus
James B, Thoden, Hazel M, Holden
openaire +2 more sources
Radiation Oncology, 2021 Purpose Stereotactic body radiotherapy (SBRT) is increasingly used for treatment of liver tumors but the effect on metabolic liver function in surrounding tissue is largely unknown.
Michael Sørensen +4 more
doaj +1 more source
Unusual Presentation of Classical Galactosemia: A Case Report of Iranian Experience. [PDF]
Clin Case RepABSTRACT Galactosemia is a rare autosomal recessive metabolic disorder with four main types, and classic galactosemia is the most prevalent. These patients have galactose‐1‐phosphate‐uridyltransferase deficiency. We report on a case of an infant who was admitted with poor feeding, lethargy, and poor weight gain.
Alaee M +4 more
europepmc +2 more sources
Insight into the mechanism of galactokinase: role of a critical glutamate residue and helix/coil transitions [PDF]
, 2016 Galactokinase, the enzyme which catalyses the first committed step in the Leloir pathway, has attracted interest due to its potential as a biocatalyst and as a possible drug target in the treatment of type I galactosemia.
Huang, Meilan +2 more
core +2 more sources
A presença da Galactoquinase em Tecidos Animais
Brazilian Archives of Biology and Technology, 2001 O autor relata trabalhos experimentais que o levaram a concluir pela existência da galactoquinase em tecidos animais, explicando desta maneira, que a primeira fase do metabolismo intermediário da galactose nos mesmos é a sua esterificação, em presença do
Metry Bacila
doaj +1 more source
Altered expression response upon repeated gene repression in single yeast cells.
PLoS Computational Biology, 2022 Cells must continuously adjust to changing environments and, thus, have evolved mechanisms allowing them to respond to repeated stimuli. While faster gene induction upon a repeated stimulus is known as reinduction memory, responses to repeated repression
Lea Schuh +6 more
doaj +1 more source
Systematic Chemoenzymatic Synthesis of O-Sulfated Sialyl Lewis x Antigens. [PDF]
, 2015 O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes.
Chen, Xi +8 more
core +1 more source