Results 141 to 150 of about 102,033 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Les acariens du genre Megninia (Analgidae), parasites de Gallus gallus [PDF]

, 1985
On peut trouver sur les poulets domestiques l'une ou l'autre de trois espèces du genre Megninia : M. cubitalis (Mégnin 1877), M. ginglymura (Megnin 1877) et M. ortari, n. sp. ici décrite.
Atyeo, W.T., Barré, Nicolas, Gaud, J.
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Content of exDNA in rooster’s seminal plasma as a potential biomarker of sperm viability

Аграрная наука Евро-Северо-Востока
Searching biomarkers, that have an early prognostic trait and determinate functional status of male gametes Gallus Gallus is a promising direction for improving ejaculate’s quality reproductive characteristics.
A. A. Kurochkin, T. I. Kuzmina, A. O. Prituzhalova   +2 more
doaj   +1 more source

Сезонные колебания миграционной активности ядерных гемоцитов позвоночных животных при разных температурах инкубации [PDF]

, 2014
В тесте миграции под агарозой установлено, что ядерные эритроциты Cyprinus carpio, Rana ridibunda и Gallus domesticus способны к спонтанным локомоциям. Процесс миграции у красных клеток крови лягушки сопряжен с образованием длинных псевдоподий, у сазана ...
Буковцова, И. С., Кует, Д. Х., Тхань, В. В., Чернявских, С. Д.   +3 more
core  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Detection of anti: Rickettsia spp. antibodies in domestic chickens of extensive breeding in an endemic area for spotted fever in the state of Rio Grande do Sul, Brazil

Ciência Rural, 2013
The goal of this study was to investigate anti-Rickettsia spp. antibodies in sera of domestic chickens (Gallus gallus domesticus) of extensive breeding in Cerro Largo county, considered an endemic area for spotted fever in the State of Rio Grande do Sul,
Jonas Fernandes Maciel, Felipe da Silva Krawczak, Caroline Sobotyk de Oliveira, Jonas Moraes-Filho, Marcelo Bahia Labruna, Sônia de Avila Botton, Fernanda Silveira Flores Vogel, Luis Antonio Sangioni   +7 more
doaj