Use of Partial Purified Lipase From local Chicken kidney for Improvement the Flavor of Butter Fat
Lipase was extracted by Sodium acetate buffer (pH=6; 0.05M) Containing 0.1M NaCl. Enzyme content of crude extract was concentrated by gradual addition of ammonium sulfate at 30-60% saturation.
A. N. Al-Hasnawi+2 more
doaj
Correlations between retention volume and molecular size parameters in gel permeation chromatography of small molecules [PDF]
Robert A. Shanks
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Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen+5 more
wiley +1 more source
Purification of rat pancreatic lipase
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj
Chromatography of Penicillins, Penicilloates, and Penicilloylamides on Dextran Gels [PDF]
Newton E. Hyslop, Richard J. Milligan
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Magnetically Guided Mechanoactive Mineralization Scaffolds for Enhanced Bone Regeneration
A 3D‐printed ‘rebar‐concrete’ inspired scaffold (PGS‐P@MGel) synergizes spontaneous biomineralization with magneto‐mechanical stimulation through PDA@Fe3O4‐embedded hydrogel. This dual biointerface activates Piezo1/β‐catenin/YAP mechanotransduction axis, enhancing BMSCs osteogenesis and angiogenesis simultaneously.
Xuran Guo+10 more
wiley +1 more source
Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography.
Pei-Pei Wang+3 more
doaj
Displacement Chromatography of Lublicating Oils by Silica Gel
Ryusuke Ono, Kozaburo Kurihara
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Use of Thin-Layer Chromatography on Silica Gel for Serum Lipid Fractionation and Measurement in the Routine Clinical Laboratory [PDF]
Antonio Chedid, P. Haux, Samuel Natelson
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Adalimumab‐Poloxamer Conjugate for Bio‐Better: Enhanced Stability and Function
Antibody‐polymer conjugates, particularly poloxamer conjugates, enhance antibody stability by improving tolerance to physicochemical stress and attenuating proteolysis by proteases. Furthermore, the higher affinity observed with poloxamer conjugation compared to standard PEGylation results in improved therapeutic efficacy in rheumatoid arthritis mouse ...
Jaewon Roh+3 more
wiley +1 more source