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Gel‐Filtration Chromatography [PDF]

open access: possibleCurrent Protocols in Molecular Biology, 1998
AbstractGel filtration (GF) chromatography separates proteins solely on the basis of molecular size. Separation is achieved using a porous matrix to which the molecules, for steric reasons, have different degrees of access‐‐i.e., smaller molecules have greater access and larger molecules are excluded from the matrix. Hence, proteins are eluted from the
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Reproducibility of gel‐chromatography

International Journal of Peptide and Protein Research, 1984
We think it worthwhile to other experimenters to mention the following observations. Recently it was noted that gel chromatographic separations of the five fragments resulting from the degradation of cytochrome c by bromocyanogen became dependent on the batch number of the gel. This dependency concerns only separations of unprotected fragments, using 7%
Tesser, G.I.   +3 more
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Gel chromatography of heparin

Journal of Chromatography B: Biomedical Sciences and Applications, 1981
It is assumed that heparin is a heterogeneous substance. In order to further investigate the purification of heparin, a column chromatographic technique for the fractionation of heparin is described using various diameters of bead form cross-linked dextran gels and an automated apparatus. It was observed that Sephadex G-50 resulted in the separation of
Ray Losito   +2 more
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Resins and gels for chromatography

Journal of Chromatography A, 1970
Abstract Ion exchangers and gels for chromatography synthesized by the authors are decribed. Resins and gels for chromatography have been prepared based on copolymers of styrene and p - or m divinylbenzene. They are characterized by their high purity, homogeneity of granular composition, high mechanical strength and stability, and the ...
S.B. Makarova, E.V. Egorov
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Gel chromatography of carbohydrates on polysaccharide gel

Journal of Polymer Science: Polymer Symposia, 1980
AbstractLiquid chromatographies of high and low‐molecular carbohydrates and polyethyleneglycols of different molecular masses were studied on porous microspherical cellulose. Dependence of the distribution coefficients of polymer molecules on molecular mass was determined.
Yu. A. Eltekov   +4 more
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Gel matrices for scanning gel chromatography

Biophysical Chemistry, 1979
I have examined the light-scattering behavior of a number of gel matrices used in gel filtration chromatography. The angular dependence of light scattering by Sephadexes is consistent with treatment of the particles as large scattering particles with a low refractive index increment (mu).
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Fluorinated gels for gel permeation chromatography

Die Makromolekulare Chemie, 1993
AbstractThe aim of these investigations was the development of stationary phases for GPC compatible with fluorinated solvents. The properties of crosslinked copolymers of 4‐(2‐heptafluoropropoxy‐1, 2,2‐trifluoroethoxy)styrene (1) were investigated in dependence of the mode of synthesis. Divinylbenzene (DVB) was used as crosslinking agent.
Hans‐Jürgen Juhl, Walter Heitz
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Gel diffusion chromatography of dextran

Journal of Chromatography A, 1962
Abstract Glucose and dextrans of molecular weight 5,000 to 300,000 can be partially separated chromatographically on columns of agar (6–9 %) with buffer (pH 8.0) or water as eluant. Agarose gels (4 %) can also be used with water as eluant. The method can be used to estimate approximately the molecular weight distribution in small samples of clinical ...
Dorothy C. Smith, Brian C.W. Hummel
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