Results 91 to 100 of about 1,334,151 (300)

Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE

BioTechniques, 2014
Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl, Xiexiong Deng, Mengyu Liu, Michael J McAndrew, Stacy Hovde, Xinjing Xu, Min-Hao Kuo   +6 more
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Disassembly of electrophoresis in polyacrylamide gel and gel staining

, 2021
For the visualization of the separation of proteins by electrophoresis in polyacrylamide gel, once the electrophoresis is performed, the gel will be taken out of the system and the proteins stained, as explained in this video.https://media.upv.es/player/?
Yenush, Lynne, Andrés-Colás, Nuria
core  

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Agarose gel electrophoresis of PCR product.

, 2013
Agarose gel electrophoresis of PCR product.
Parasakthi Navaratnam (378400), Lip Yong Chung (378399), Pooi Yin Chung (378398)   +2 more
core   +1 more source

A Physical Map Including a New Class I Gene (cda12) of the Human Major Histocompatibility Complex (A2/313 Haplotype) Derived from a Monosomy 6 Mutant Cell Line [PDF]

, 1989
To avoid interpretative problems due to restriction fragment length polymorphisms, the monosomy 6 mutant cell line BM19.7 was employed to establish a molecular map of the human major histocompatibility (HLA) complex in the A2,B13,Bw4,DRw6,DRw52,DQw1,DPw2
Pohla, Heike, Messer, Gerald, Bloemer, Katharina, Ziegler, Andreas, Ragoussis, Jiannis, Weiss, Elisabeth H.   +5 more
core   +1 more source

Gel Diffusiophoresis of a Spherical Colloidal Particle

Fluids
A theoretical framework is established for the gel diffusiophoresis of a spherical colloidal particle moving through an uncharged dilute porous polymer gel medium when an electrolyte concentration gradient field is applied.
Hiroyuki Ohshima
doaj   +1 more source

Protection of DNA During Preparative Agarose Gel Electrophoresis Against Damage Induced by Ultraviolet Light

BioTechniques, 1996
Preparative gel electrophoresis of double- stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in
Dirk Gründemann, Edgar Schömig
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Gel-eletroforese no diagnóstico da varíola Gel-electrophoresis in the smallpox diagnosis

Memorias do Instituto Oswaldo Cruz, 1972
O emprego de gel-eletroforese no diagnóstico da varíola, demonstrou ser ao menos trinta vezes (30X) mais sensível que o teste de agar-gel, nas condições descritas (tabela I).
Julio A. Mesquita, Akira Homma, Hermann G. Schatzmayr   +2 more
doaj   +1 more source