Results 111 to 120 of about 1,334,151 (300)

Detection of rotavirus genome by new silver staining method

open access: yesمجله دانشگاه علوم پزشکی گرگان, 2014
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj  

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

open access: yesFEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Pulsed-Field Gel Electrophoresis patterns

open access: yes
The fingerprints that scientist use to identify bacteria are called Pulsed-Field Gel Electrophoresis (PFGE).Part of series 3 of the CDC Museum set of Infectious disease trading cards, featuring photos and information about some of the infectious diseases

core  

PHYLOGENETIC ANALYSIS OF BACTERIAL COMMUNITIES IN PANCURAN 7 BATURRADEN HOT SPRING [PDF]

open access: yes, 2006
The diversity of the bacterial communities supported by culturing and capturing through 0.2 m-pore-size filter was studied. The Pancuran 7 hot spring has temperature at around 52°C and pH 7.
Mulyani, N.S.   +5 more
core   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

open access: yesFEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi   +6 more
wiley   +1 more source

A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes : towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

open access: yes, 1991
A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established.
Rasmussen, Hanne H   +24 more
core   +1 more source

Agarose gel electrophoresis: Applications in clinical chemistry [PDF]

open access: yesJournal of Medical Biochemistry, 2010
Agarose gel electrophoresis is a well established technique routinely used in clinical laboratories for screening protein abnormalities in various biological fluids (serum, urine, CSF).
Giot Jean-Francois
doaj  

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

open access: yesFEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li   +7 more
wiley   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

open access: yesFEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli   +2 more
wiley   +1 more source

Bi‐ and Mono‐Allelic RFC1 Expansion in a North American Cohort With Idiopathic Axonal Neuropathy

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective RFC1 biallelic repeat expansion is increasingly recognized as a cause of chronic idiopathic axonal polyneuropathy (CIAP), but it remains challenging to know who to test. This study aims to determine the prevalence of biallelic and monoallelic RFC1 expansions and their corresponding neuropathy phenotypes in CIAP patients and identify ...
Amro M. Stino   +25 more
wiley   +1 more source

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