Results 271 to 280 of about 1,713,569 (328)
Some of the next articles are maybe not open access.
Difference gel electrophoresis
ELECTROPHORESIS, 2009AbstractDifference gel electrophoresis (DIGE) was invented to circumvent the inherent variability of 2‐DE. This variability is a natural consequence of separating thousands of proteins over a large space, such as a 15×20 cm slab of polyacrylamide gel.
Jonathan S, Minden +3 more
openaire +2 more sources
Clinica Chimica Acta, 1967
Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
U, Krause, V, Raunio
openaire +2 more sources
Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
U, Krause, V, Raunio
openaire +2 more sources
Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
A, Chrambach, D, Rodbard
openaire +2 more sources
Analysis of DNA by Agarose Gel Electrophoresis.
Cold Spring Harbor Protocols, 2019Electrophoresis through agarose or polyacrylamide gels is used to separate, analyze, identify, and purify DNA fragments. The technique is simple, rapid to perform, and capable of resolving fragments of DNA that cannot be separated adequately by other ...
Michael R Green, J. Sambrook
semanticscholar +1 more source
Carbohydrate Gel Electrophoresis
2010Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation ...
Goubet, Florence +2 more
openaire +5 more sources
Difference gel electrophoresis
PROTEOMICS, 2008AbstractDIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical ...
John F, Timms, Rainer, Cramer
openaire +2 more sources
Current Protocols in Molecular Biology, 1992
AbstractAgarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5‐ to 25‐kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample ...
openaire +5 more sources
AbstractAgarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5‐ to 25‐kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample ...
openaire +5 more sources
Difference gel electrophoresis DIGE
Drug Discovery Today: Technologies, 2006Proteomics offers powerful technologies to assist in the discovery of targets for novel therapeutic agents, by allowing the investigation of changes in protein state between control and diseased tissue and biofluids. Difference gel electrophoresis coupled with mass spectrometry (DIGE/MS) is a technology used within proteomics that has demonstrated ...
Kathryn S, Lilley, David B, Friedman
openaire +2 more sources
Trends in Biochemical Sciences, 2000
Work at Brookhaven National Laboratory during the period described in the article was supported by the US Atomic Energy Commission, a predecessor to our current sponsor, the US Dept of Energy.
openaire +2 more sources
Work at Brookhaven National Laboratory during the period described in the article was supported by the US Atomic Energy Commission, a predecessor to our current sponsor, the US Dept of Energy.
openaire +2 more sources
2003
After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
openaire +3 more sources
After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
openaire +3 more sources