Results 281 to 290 of about 1,713,569 (328)
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Current Protocols in Immunology, 1991
AbstractDiagonal gel electrophoresis is a form of two‐dimensional analysis useful for investigating the subunit composition of multisubunit proteins containing interchain disulfide bonds. Proteins are electrophoresed in the first dimension in a slab or tube gel under nonreducing conditions. The proteins are then reduced in the gel and this piece of gel
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AbstractDiagonal gel electrophoresis is a form of two‐dimensional analysis useful for investigating the subunit composition of multisubunit proteins containing interchain disulfide bonds. Proteins are electrophoresed in the first dimension in a slab or tube gel under nonreducing conditions. The proteins are then reduced in the gel and this piece of gel
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Pulsed-field gel electrophoresis
Molecular Biotechnology, 1998Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive ...
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2013
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
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Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
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2022
Gel electrophoresis is used to separate and analyze biomolecules, such as DNA, RNA, and proteins. By applying an electric field to a gel matrix, charged molecules migrate at varying speeds based on their size and charge. This process enables researchers to visualize and quantify nucleic acids and proteins, making it an essential tool for genetic ...
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Gel electrophoresis is used to separate and analyze biomolecules, such as DNA, RNA, and proteins. By applying an electric field to a gel matrix, charged molecules migrate at varying speeds based on their size and charge. This process enables researchers to visualize and quantify nucleic acids and proteins, making it an essential tool for genetic ...
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Electrophoresis in gel channels
ELECTROPHORESIS, 2005AbstractWe describe a novel approach to generate dynamic pH gradients suited to fractionate or purify samples of biomolecules or particles such as proteins and viruses in tiny volumes. The method combines diffusion and electromigration between micro‐scaled channels embedded in hydrogel.
Rolf, Hagedorn +5 more
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Conformation Sensitive Gel Electrophoresis
2005Conformation sensitive gel electrophoresis (CSGE) is a rapid screening method for the detection of DNA sequence variation, specifically single-base changes or small insertions and deletions. It has been widely used for mutation screening in genetic disorders and for the detection of single nucleotide polymorphisms (SNPs).CSGE is a simple manual method,
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Noise‐enhanced gel electrophoresis
ELECTROPHORESIS, 2014Macromolecules confined within a nanoporous matrix experience entropic trapping when their dimensions approach the average pore size, leading to emergence of anomalous transport behavior that can be beneficial in separation applications. But the ability to exploit these effects in practical settings (e.g., electrophoretic separation of DNA) has been ...
Nan, Shi, Victor M, Ugaz
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
Journal of Molecular Biology, 1975E. Southern
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Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.
Analytical Biochemistry, 1966C. Laurell
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