Gel versus capillary electrophoresis genotyping for categorizing treatment outcomes in two anti-malarial trials in Uganda [PDF]
, 2010 Background Molecular genotyping is performed in anti-malarial trials to determine whether recurrent parasitaemia after therapy represents a recrudescence (treatment failure) or new infection.
Vinay Gupta +4 more
core +1 more source
Rubisco : easy purification and immunochemical determination [PDF]
, 2001 Rubisco (Ribulose-1.5-bisphosphate carboxylase/oxygenase) from spinach was purified to homogeneity in one step by gel filtration. This enzyme is suitable for the generation of a specific antibody in rabbits.
Groß, Ulrich
core +1 more source
Use of capillary electrophoresis as a method development tool for classical gel electrophoresis [PDF]
, 1998 Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan.
Gratz, Samuel R. +2 more
core +1 more source
Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]
, 1993 The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
A.A. Friedl +39 more
core +1 more source
Analysis of proteins from marine molluscs [PDF]
, 2009 Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can
Bebianno, Maria João +2 more
core +1 more source
Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]
, 2006 Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C +3 more
core +2 more sources
Genetic Differentiation Among Three Species of \u3ci\u3eParadosa\u3c/i\u3e (Arachnida: Lycosidae) [PDF]
, 2017 Allozymic variation in nine protein producing loci was examined in three species of Pardosa using starch gel electrophoresis. Allozyme frequencies showed a high degree of geographic uniformity among conspecific populations. Estimated heterozygosities for
Elliott, N. C +3 more
core +2 more sources
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Manual for starch gel electrophoresis: A method for the detection of genetic variation [PDF]
, 1987 The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts ...
Aebersold, Paul B. +4 more
core
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source