Results 41 to 50 of about 1,334,151 (300)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat [PDF]

, 2010
Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough.
Branlard, Gerard, Rogers, William John, Liu, Li, Ikeda, Tatsuya M, Wang Aili, Yoshida, H., Branlard, G., Xia, X., Kolman, Maía de Los Angeles, Roberto J Peña, Ma, Wujun, Ma, W., Rogers, William J, Wang, A., Lerner, S.E., Gerard Branlard, Zhonghu He, Lerner, Silvia E., He, Zhonghu, Yan, Y., Wang Linhai, Linhai Wang, Lerner Silvia E, Wang, Aili, He, Z., Silvia E Lerner, Rogers William J, Wujun Ma, Branlard, Gerard,, Peña, R., Kolman María A, Xia Xianchun, William J Rogers, Appels, R., Kolman, María A, Yoshida Hisashi, Kolman, M.A., Peña, Roberto J, Yoshida, Hisashi, Rogers, W.J., Liu, L., Lerner, Silvia E, Tatsuya M Ikeda, Ma Wujun, Branlard, Gerard, G., Wang, L., Yan, Yueming, Wang, Linhai, Appels, Rudi, Kolman, Maria de Los Angeles, Hisashi Yoshida, Appels Rudi, He Zhonghu, Peña Roberto J, Liu Li, Aili Wang, Ikeda Tatsuya M, María A Kolman, Peña, R.J., Li Liu, Yan Yueming, Xia, Xianchun, Ikeda, T.M., Yueming Yan, Rogers, John William, Xianchun Xia, Branlard Gerard, Rudi Appels, Ikeda, Tatsuya M., Peña, Roberto J.   +69 more
core   +1 more source

Comparative 2-DE protein analysis in a 3-D geometry gel

BioTechniques, 2007
Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput.
Robert Ventzki, Sabrina Rüggeberg, Stefan Leicht, Thomas Franz, Josef Stegemann   +4 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Agar gel electrophoresis of amylases [PDF]

, 1969
Agar gel electrophoresis of ...
Fass, D. N., D. N. Fass
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Investigation of Gel Electrophoresis Stains

Al-SalamJournal for Medical Science
Gel electrophoresis is essential in molecular biology and biochemistry for separating and analyzing DNA, RNA, and proteins. UV sensitivity and compatibility have led to the use of ethidium bromide stains.
ibrahim ramzi hadi, Dilara Bozdogan, Ali Ahmed Kamal Khedher Agha, Ruaa Hussein Ali, Safa Al-Shattawi   +4 more
doaj   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Analysis of Serpulina hyodysenteriae strain variation and its molecular epidemiology using pulsed-field gel electrophoresis [PDF]

, 1999
Pulsed-field gel electrophoresis (PFGE) was applied as a molecular typing tool for the spirochaete Serpulina hyodysenteriae, the agent of swine dysentery.
Atyeo, R.F., Oxberry, S.L., Hampson, D.J.   +2 more
core  

ARTGEL: A temperature-regulated gel electrophoresis platform for quantitative studies of reversible association

HardwareX
Here we present ARTGEL, an actively regulated-temperature gel electrophoresis platform designed for quantitative studies of temperature-sensitive reversible biomolecular association.
Rupam Saha, Seth Fraden
doaj   +1 more source