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Angle modulated two-dimensional single cell pulsed-field gel electrophoresis for detecting early symptoms of DNA fragmentation in human sperm nuclei. [PDF]
Sci RepKaneko S, Takamatsu K.
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Scandinavian Journal of Clinical and Laboratory Investigation, 1972
A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described.
B. Johansson
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A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described.
B. Johansson
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Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2020Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R, Green, Joseph, Sambrook
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Electrophoresis in gel channels
ELECTROPHORESIS, 2005AbstractWe describe a novel approach to generate dynamic pH gradients suited to fractionate or purify samples of biomolecules or particles such as proteins and viruses in tiny volumes. The method combines diffusion and electromigration between micro‐scaled channels embedded in hydrogel.
Rolf, Hagedorn +5 more
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Nanocellulose for gel electrophoresis
Journal of Colloid and Interface Science, 2019Cellulose nanofibres produced by TEMPO-mediated oxidation can form gels. This study presents a proof-of-concept for gel electrophoresis with nanocellulose (NC).TEMPO-oxidised cellulose nanofibre dispersion is chemically cross-linked by inducing amide linkages to produce gel slabs for electrophoretic separation.
Llyza, Mendoza +3 more
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Difference gel electrophoresis
ELECTROPHORESIS, 2009AbstractDifference gel electrophoresis (DIGE) was invented to circumvent the inherent variability of 2‐DE. This variability is a natural consequence of separating thousands of proteins over a large space, such as a 15×20 cm slab of polyacrylamide gel.
Jonathan S, Minden +3 more
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The gel electrophoresis of DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract 1. 1. We have compared the electrophoretic mobility of linear duplex DNAs (bacteriophages T4, lambda, T7 and Φ29) and of circular duplex DNAs (replicative form DNA of bacteriophage ΦX174, bacteriophage PM 2 DNA and rat mtDNA) in agarose gels.
C, Aaij, P, Borst
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Analysis of DNA by Agarose Gel Electrophoresis.
Cold Spring Harbor Protocols, 2019Electrophoresis through agarose or polyacrylamide gels is used to separate, analyze, identify, and purify DNA fragments. The technique is simple, rapid to perform, and capable of resolving fragments of DNA that cannot be separated adequately by other ...
Michael R Green, J. Sambrook
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Clinica Chimica Acta, 1967
Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
U, Krause, V, Raunio
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Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
U, Krause, V, Raunio
openaire +2 more sources