Results 311 to 320 of about 1,126,750 (353)
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Cold Spring Harbor Protocols, 2006
This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
David W. Russell, Joseph Sambrook
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This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
David W. Russell, Joseph Sambrook
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Difference gel electrophoresis
ELECTROPHORESIS, 2009AbstractDifference gel electrophoresis (DIGE) was invented to circumvent the inherent variability of 2‐DE. This variability is a natural consequence of separating thousands of proteins over a large space, such as a 15×20 cm slab of polyacrylamide gel.
Kai Stühler +3 more
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Electrophoresis in gel channels
ELECTROPHORESIS, 2005AbstractWe describe a novel approach to generate dynamic pH gradients suited to fractionate or purify samples of biomolecules or particles such as proteins and viruses in tiny volumes. The method combines diffusion and electromigration between micro‐scaled channels embedded in hydrogel.
Kristian Lange +5 more
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Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2020Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R. Green, Joseph Sambrook
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Carbohydrate Gel Electrophoresis
2010Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation ...
Goubet, Florence +2 more
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The gel electrophoresis of DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract 1. 1. We have compared the electrophoretic mobility of linear duplex DNAs (bacteriophages T4, lambda, T7 and Φ29) and of circular duplex DNAs (replicative form DNA of bacteriophage ΦX174, bacteriophage PM 2 DNA and rat mtDNA) in agarose gels.
C. Aaij, Piet Borst
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Nanocellulose for gel electrophoresis
Journal of Colloid and Interface Science, 2019Cellulose nanofibres produced by TEMPO-mediated oxidation can form gels. This study presents a proof-of-concept for gel electrophoresis with nanocellulose (NC).TEMPO-oxidised cellulose nanofibre dispersion is chemically cross-linked by inducing amide linkages to produce gel slabs for electrophoretic separation.
Gil Garnier +3 more
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Current Protocols in Molecular Biology, 1992
AbstractAgarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5‐ to 25‐kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample ...
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AbstractAgarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5‐ to 25‐kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample ...
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Clinica Chimica Acta, 1967
Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
V. Raunio, U. Krause
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Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
V. Raunio, U. Krause
openaire +3 more sources