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Current Protocols in Immunology, 1991
AbstractDiagonal gel electrophoresis is a form of two‐dimensional analysis useful for investigating the subunit composition of multisubunit proteins containing interchain disulfide bonds. Proteins are electrophoresed in the first dimension in a slab or tube gel under nonreducing conditions. The proteins are then reduced in the gel and this piece of gel
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AbstractDiagonal gel electrophoresis is a form of two‐dimensional analysis useful for investigating the subunit composition of multisubunit proteins containing interchain disulfide bonds. Proteins are electrophoresed in the first dimension in a slab or tube gel under nonreducing conditions. The proteins are then reduced in the gel and this piece of gel
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Quantitative Gel Electrophoresis
2018Gel electrophoresis is the most widely used technique for the analysis of protein samples, and there are a variety of methods that can be used to investigate single proteins as well as highly complex protein mixtures. To ensure reproducible and reliable separations of such samples and the resolution of distinct protein species, there has been a ...
Gauci-Mansour, Victoria J. (R17185) +1 more
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Two‐Dimensional Gel Electrophoresis
Current Protocols in Protein Science, 1998AbstractTwo‐dimensional gel electrophoresis combines two different electrophoretic separating techniques in perpendicular directions to provide a much greater separation of complex protein mixtures than either of the individual procedures. Variations of the most common two‐dimensional technique are described in this unit, namely isoelectrofocusing (IEF)
David W. Speicher +2 more
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Two‐Dimensional Gel Electrophoresis
Current Protocols in Immunology, 2004AbstractTwo‐dimensional gel electrophoresis is the combination of two high‐resolution electrophoretic procedures (isoelectric focusing and SDS‐polyacrylamide gel electrophoresis) to provide much greater resolution than either procedure alone. In the first‐dimension gel, solubilized proteins are separated according to their isoelectric point (pI) by ...
Lonnie D. Adams, Sean R. Gallagher
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Current Protocols Essential Laboratory Techniques, 2008
AbstractAgarose gel electrophoresis, which separates and sizes linear DNA and RNA fragments, is arguably the most basic and essential technique in molecular biology. It is commonly employed for analysis of PCR products, plasmid DNA, and products of restriction enzyme digestion.
Jennifer A. Armstrong, Joseph R. Schulz
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AbstractAgarose gel electrophoresis, which separates and sizes linear DNA and RNA fragments, is arguably the most basic and essential technique in molecular biology. It is commonly employed for analysis of PCR products, plasmid DNA, and products of restriction enzyme digestion.
Jennifer A. Armstrong, Joseph R. Schulz
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ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS
Canadian Journal of Biochemistry and Physiology, 1963Histories extracted from various organs of the rat have been fractionated by electrophoresis on Polyacrylamide gels. The most convenient reagents to form the gel were selected and the effects of varying concentrations of these reagents on the resolution of histones were investigated.
A. M. Marko, L. D. Johnson, A. Driedger
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2013
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
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Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
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2017
Gel electrophoresis (GE) is a versatile tool for research on viroids and other small circular nucleic acids. GE systems can be adapted to suit specific applications by adjusting variables of matrix and running conditions. Since sequence information is not required, GE is suitable for identifying that a viroid is associated with a disease of unknown ...
D. Hanold, Ganesan Vadamalai
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Gel electrophoresis (GE) is a versatile tool for research on viroids and other small circular nucleic acids. GE systems can be adapted to suit specific applications by adjusting variables of matrix and running conditions. Since sequence information is not required, GE is suitable for identifying that a viroid is associated with a disease of unknown ...
D. Hanold, Ganesan Vadamalai
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Conformation Sensitive Gel Electrophoresis
2005Conformation sensitive gel electrophoresis (CSGE) is a rapid screening method for the detection of DNA sequence variation, specifically single-base changes or small insertions and deletions. It has been widely used for mutation screening in genetic disorders and for the detection of single nucleotide polymorphisms (SNPs).CSGE is a simple manual method,
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