Results 91 to 100 of about 3,553,338 (335)
Advanced glycation end products promote the release of endothelial cell‐derived mitocytosis
Under diabetic conditions, AGEs induce mitochondrial damage in HUVECs, activating migrasome‐mediated mitocytosis. Migrasomes encapsulate damaged mitochondria and are released into the extracellular space, facilitating intercellular mitochondrial transfer.
Rong Liu+6 more
wiley +1 more source
Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.
Brittney M. Howard+3 more
doaj
A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation ...
G. Rotková
doaj +1 more source
Two-dimensional profiling of proteins from Curculigo latifolia fruit by three different extraction protocols. [PDF]
Lemba, Curculigo latifolia (family Hypoxidaceae) is an indigenous plant of Malaysia, found mainly in swampy areas of tropical Asia and Australia. This study was designed to compare the efficiency of three protocols employed for the extraction of ...
Ahmad, Zalinah+3 more
core
Silver-staining of proteins in polyacrylamide gels: a general overview [PDF]
On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested.
arxiv
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna+4 more
wiley +1 more source
Hierarchical transformation-guided image registration for two-dimensional gel electrophoresis image
Many proteomics studies have been devoted to investigate the gel-based methods for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens.
Lifang Wei+5 more
doaj +1 more source
Western and dot immunoblotting analysis of viral antigens and antibodies: application to murine hepatitis virus. [PDF]
Viral proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred quantitatively to nitrocellulose by electroblotting in SDS-containing buffer.
Buchmeier, MJ, Knobler, RL, Talbot, PJ
core
The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise
Frailty syndrome exacerbates skeletal muscle degeneration via increased ECM deposition and myofiber loss. This study, using a murine model, demonstrates that endurance exercise attenuates these histopathological alterations, preserving muscle integrity. Findings support exercise as a viable strategy to counteract frailty‐induced musculoskeletal decline
Fujue Ji+3 more
wiley +1 more source
The inactivation of SLC35C1 (GDP‐fucose transporter) and enzymes involved in GDP‐fucose biosynthesis was studied. Fucose supplementation increases the level of GDP‐fucose to abnormal, millimolar values in the absence of the TSTA3 protein and SLC35C1 in contrast to the GMDS/SLC35C1 double mutant.
Edyta Skurska, Mariusz Olczak
wiley +1 more source