Results 91 to 100 of about 864,514 (341)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements

Data in Brief, 2016
In this article we present data on the concentration of calcium as determined by Inductively Coupled Plasma (ICP) measurements. Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor (NCS) proteins.
Jeffrey Viviano, Hao Wu, Anuradha Krishnan, Kandalam Ramanujachary, Venkat Venkataraman   +4 more
doaj   +1 more source

Hierarchical transformation-guided image registration for two-dimensional gel electrophoresis image

Journal of Algorithms & Computational Technology, 2020
Many proteomics studies have been devoted to investigate the gel-based methods for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens.
Lifang Wei, Heng Dong, Changcai Yang, Zhe Guan, Wenfang Lin, Ying Miao   +5 more
doaj   +1 more source

B-cell chronic lymphocytic leukemia-associated nuclear antigens [PDF]

, 2008
One- and two-dimensional polyacrylamide gel electrophoresis were used to compare the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells.
Błoński, Jerzy Z., Kiliańska, Zofia M., Robak, Tadeusz, Rogalinska, Malgorzata   +3 more
core  

Silver Staining of Proteins in 2DE Gels [PDF]

, 2009
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals.
Chevallet, Mireille, Lelong, Cécile, Luche, Sylvie, Rabilloud, Thierry   +3 more
core   +5 more sources

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Comparative 2-DE protein analysis in a 3-D geometry gel

BioTechniques, 2007
Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput.
Robert Ventzki, Sabrina Rüggeberg, Stefan Leicht, Thomas Franz, Josef Stegemann   +4 more
doaj   +1 more source

Analysis of proteins from marine molluscs [PDF]

, 2009
Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can
Bebianno, Maria João, Chora, Suze, Roméo, Michèle   +2 more
core   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Osmotic pressure: resisting or promoting DNA ejection from phage [PDF]

, 2008
Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I on the course of ejection.
Castelnovo, Martin, Evilevitch, Alex,, Jeembaeva, Meerim,, Larsson, Frida, Lund University.   +4 more
core   +3 more sources