Results 111 to 120 of about 929,641 (371)

Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements

Data in Brief, 2016
In this article we present data on the concentration of calcium as determined by Inductively Coupled Plasma (ICP) measurements. Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor (NCS) proteins.
Jeffrey Viviano, Hao Wu, Anuradha Krishnan, Kandalam Ramanujachary, Venkat Venkataraman   +4 more
doaj   +1 more source

Hierarchical transformation-guided image registration for two-dimensional gel electrophoresis image

Journal of Algorithms & Computational Technology, 2020
Many proteomics studies have been devoted to investigate the gel-based methods for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens.
Lifang Wei, Heng Dong, Changcai Yang, Zhe Guan, Wenfang Lin, Ying Miao   +5 more
doaj   +1 more source

Inhibition of CDK9 enhances AML cell death induced by combined venetoclax and azacitidine

Molecular Oncology, EarlyView.
The CDK9 inhibitor AZD4573 downregulates c‐MYC and MCL‐1 to induce death of cytarabine (AraC)‐resistant AML cells. This enhances VEN + AZA‐induced cell death significantly more than any combination of two of the three drugs in AraC‐resistant AML cells.
Shuangshuang Wu, Jianlei Zhao, Aaban Asfar Azmi, Avanti Gupte, Jenna Thibodeau, Shuang Liu, Jinli Yang, Guan Wang, Holly Edwards, Lisa A. Polin, Juiwanna Kushner, Sijana H. Dzinic, Kathryn White, Julie Boerner, Maik Hüttemann, Jay Yang, Yue Wang, Jeffrey W. Taub, Yubin Ge   +18 more
wiley   +1 more source

Reconstitution of recombination-associated DNA synthesis with human proteins. [PDF]

, 2013
The repair of DNA breaks by homologous recombination is a high-fidelity process, necessary for the maintenance of genome integrity. Thus, DNA synthesis associated with recombinational repair must be largely error-free.
Grossi, Sara M, Heyer, Wolf-Dietrich, Hurwitz, Jerard, Sneeden, Jessica L, Tappin, Inger   +4 more
core   +1 more source

Comparative 2-DE protein analysis in a 3-D geometry gel

BioTechniques, 2007
Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput.
Robert Ventzki, Sabrina Rüggeberg, Stefan Leicht, Thomas Franz, Josef Stegemann   +4 more
doaj   +1 more source

Characterizing epithelial‐mesenchymal transition‐linked heterogeneity in breast cancer circulating tumor cells at a single‐cell level

Molecular Oncology, EarlyView.
In over 50% of non‐metastatic breast cancer patients, circulating tumor cells (CTCs) along the whole epithelial‐mesenchymal transition spectrum are detected. Total CTC number and individual phenotypes relate to aggressive disease characteristics, including lymph node involvement and higher tumor proliferation. At the single‐cell level, mesenchymal CTCs
Justyna Topa, Julia Richert, Tomasz Stokowy, Alicja Staśczak, Mariusz Szajewski, Maciej Ciesielski, Petra M. Grešner, Bartłomiej Tomasik, Łukasz Arcimowicz, Agnieszka Stankiewicz, Grażyna Suchodolska, Elżbieta Senkus, Wiesław Kruszewski, Anna J. Żaczek, Aleksandra Markiewicz   +14 more
wiley   +1 more source

PROTEOMIC ANALYSIS OF TWO DIFFERENT STATES OF NAEGLERIA FOWLERI [PDF]

, 2011
Naegleria fowleri are free-living ameboflagellates found in soil and freshwater habitats throughout the world that cause a fatal disease in humans called Primary Amoebic Meningoencephalitis (PAM).
Park, Hong
core   +1 more source

Heterogeneity of wheat endosperm proteolipids (CM proteins) [PDF]

, 1975
Proteins extracted with CHCl3-MeOH from wheat endosperm have been fractionated by Sephadex G-100 and the 15 000–20 000 MW range fraction, designated CM protein, has been examined by combined electrofocusing (pH range 5–8) and electrophoresis (pH 3.2) and
Aragoncillo, Bietz, C. Aragoncillo, Charbonnier, Drysdale, F. Garcia-Olmedo, Folch, Folch-Pi, Garcia, Garcia, Hall, Huebner, Johnson, Johnson, Jones, Kaltsikes, M.A. Rodriguez-Loperena, Meredith, Meredith, Pilar Carbonero, Redman, Rohrlich, Tennenbaum, Waines, Waines, Wrigley, Wrigley, Zill   +27 more
core   +2 more sources

Utility of immunofixation in complementing and empowering serum protein electrophoresis in the diagnosis of paraproteinemia: Experience at a tertiary care center [PDF]

Medical Laboratory Journal
Background: ‘M’ proteins or paraproteins refer to immunoglobulins that are produced by clonal plasma cells and are a characteristic feature of monoclonal gammopathies.
Imran Ahmed Siddiqui, Sowmya Gayatri C, Swati Suravaram, Bharat Kumar Reddy, Dhanalakshmi A   +4 more
doaj  

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source