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Two-dimensional gel electrophoresis of membrane proteins
Biochemistry, 1976A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G F, Ames, K, Nikaido
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Gel Electrophoresis of Proteins
1998Abstract This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis ...
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Resolving Proteins for Immunoblotting by Gel Electrophoresis
Cold Spring Harbor Protocols, 2018This protocol describes Tris/glycine SDS–polyacrylamide gel electrophoresis, also known as SDS discontinuous gel electrophoresis or the Laemmli electrophoresis system. The gel-casting unit is assembled and tested to make sure that there are no leaks. Ammonium persulfate and tetramethylethylenediamine are added to the separating monomer solution, and ...
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Gel electrophoresis in studies of protein conformation and folding
Analytical Biochemistry, 1984Electrophoresis through polyacrylamide gels is a useful method for distinguishing conformational states of proteins and analyzing the thermodynamic and kinetic properties of transitions between conformations. Although the relationship between protein conformation and electrophoretic mobility is quite complex, relative mobilities provide qualitative ...
D P, Goldenberg, T E, Creighton
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Polyacrylamide gel electrophoresis of myelin proteins: A caution
Analytical Biochemistry, 1975Abstract Some variables involved in the preparation of rat brain myelin proteins for polyacrylamide gel electrophoresis in buffers containing sodium dodecyl sulfate were studied. Under mild conditions of solubilization the resultant gel patterns were relatively insensitive to the β-mercaptoethanol (ME) concentration in the protein solvent used for ...
P, Morell, R C, Wiggins, M J, Gray
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Separation of olive proteins by capillary gel electrophoresis
Talanta, 2012Olive proteins are not well known and there are still a lot of unknown information requiring further studies focused on the determination and characterization of these proteins. Despite the widely use of gel electrophoresis, this is the first time that capillary gel electrophoresis (CGE) is applied to separate proteins extracted from olive fruits ...
Cristina, Montealegre +4 more
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Measurement of proteins separated by electrophoresis in starch gel
Clinica Chimica Acta, 1960Abstract Quantitative methods for the measurement of proteins separated by electrophoresis in starch gel have been investigated. Scanning transparent stained sections with a specially constructed scanner gave the same results as elution of protein-bound dye. Reproducible results were obtained when normal human serum protein patterns were scanned.
H J, RUBINSTEIN, J A, OWEN, W, LARSEN
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Studies of DNA‐protein interactions by gel electrophoresis
ELECTROPHORESIS, 1989AbstractThe use of gel electrophoresis in studies of nucleic acid‐protein (especially DNA‐protein) interactions has yielded much qualitative and quantitative information about a variety of such systems. The reduction in mobility of complexes relative to free DNA allows isolation and characterization of the complexes as well as determination of ...
J A, Ceglarek, A, Revzin
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Gel Electrophoresis of Proteins and Enzymes
1976Proteins are defined as large molecular weight substances (104 – 105) composed of amino acids coupled by the α carboxyl group of one acid and the α amino group of another forming a polypeptide:
P. P. Feret, F. Bergmann
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Determination of protein phosphorylation by polyacrylamide gel electrophoresis
Journal of Microbiology, 2019Phosphorylation is the most important modification for protein regulation; it controls many signal transduction pathways in all organisms. While several tools to detect phosphorylated proteins have been developed to study a variety of basic cellular processes involving protein phosphorylation, these methods have several limitations.
Chang-Ro, Lee +4 more
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