Results 271 to 280 of about 855,961 (311)
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Resolving Proteins for Immunoblotting by Gel Electrophoresis
Cold Spring Harbor Protocols, 2018This protocol describes Tris/glycine SDS–polyacrylamide gel electrophoresis, also known as SDS discontinuous gel electrophoresis or the Laemmli electrophoresis system. The gel-casting unit is assembled and tested to make sure that there are no leaks. Ammonium persulfate and tetramethylethylenediamine are added to the separating monomer solution, and ...
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Polyacrylamide gel electrophoresis of myelin proteins: A caution
Analytical Biochemistry, 1975Abstract Some variables involved in the preparation of rat brain myelin proteins for polyacrylamide gel electrophoresis in buffers containing sodium dodecyl sulfate were studied. Under mild conditions of solubilization the resultant gel patterns were relatively insensitive to the β-mercaptoethanol (ME) concentration in the protein solvent used for ...
P, Morell, R C, Wiggins, M J, Gray
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Separation of olive proteins by capillary gel electrophoresis
Talanta, 2012Olive proteins are not well known and there are still a lot of unknown information requiring further studies focused on the determination and characterization of these proteins. Despite the widely use of gel electrophoresis, this is the first time that capillary gel electrophoresis (CGE) is applied to separate proteins extracted from olive fruits ...
Cristina, Montealegre +4 more
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Measurement of proteins separated by electrophoresis in starch gel
Clinica Chimica Acta, 1960Abstract Quantitative methods for the measurement of proteins separated by electrophoresis in starch gel have been investigated. Scanning transparent stained sections with a specially constructed scanner gave the same results as elution of protein-bound dye. Reproducible results were obtained when normal human serum protein patterns were scanned.
H J, RUBINSTEIN, J A, OWEN, W, LARSEN
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Determination of protein phosphorylation by polyacrylamide gel electrophoresis
Journal of Microbiology, 2019Phosphorylation is the most important modification for protein regulation; it controls many signal transduction pathways in all organisms. While several tools to detect phosphorylated proteins have been developed to study a variety of basic cellular processes involving protein phosphorylation, these methods have several limitations.
Chang-Ro, Lee +4 more
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Gel Electrophoresis of Proteins and Enzymes
1976Proteins are defined as large molecular weight substances (104 – 105) composed of amino acids coupled by the α carboxyl group of one acid and the α amino group of another forming a polypeptide:
P. P. Feret, F. Bergmann
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Artifacts and Common Errors in Protein Gel Electrophoresis
2018In spite of taking precautions, some common mistakes creep into well-planned gel electrophoresis experiments. This occurs commonly in relation to calculating the cross-linking factor of a gel, polymerization temperature and time for a polyacrylamide gel, inducing aggregates in samples for electrophoresis, titrating the running buffer in electrophoresis,
Biji T, Kurien, R Hal, Scofield
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Starch-gel Electrophoresis of Pig Serum Proteins
Nature, 1963MANY variations of the original technique of Smithies1 for starch-gel electrophoresis of proteins have been developed, notably the vertical gel2, the discontinuous buffer system3, and two-dimensional applications4,5. The latter method has been used by Ashton6 in examining pig serum proteins; it has one disadvantage in being time-consuming, requiring ...
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Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances
TrAC - Trends in Analytical Chemistry, 2023László Hajba +2 more
exaly