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Acetic Acid-Urea Polyacrykunide Gel Electrophoresis of Proteins
2003In SDS polyacrylamide gel electrophoresis, proteins are separated essentially on the basis of their sizes, by the sieving effect of the polyacrylamide gel matrix (see Chapter 6 ). In the absence of SDS, the proteins would still be subject to the sieving effect of the gel matrix, but their charges would vary according to their amino acid content.
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Gel Electrophoresis of Proteins and Enzymes
1976Proteins are defined as large molecular weight substances (104 – 105) composed of amino acids coupled by the α carboxyl group of one acid and the α amino group of another forming a polypeptide:
P. P. Feret, F. Bergmann
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Gel electrophoresis of protein–DNA complexes
Nature, 1987Regions of DNA bending can be explored using gel electrophoresis on protein-DNA complexes, proving gel electrophoresis does more than just separate proteins and nucleic acids according to size.
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Polyacrylamide Gel Electrophoresis of Plant Proteins
1974Development is characterized by an ordered, controlled sequence of changes that occur within an organism at the molecular, cell, tissue and organ levels. Differentiation of cells and tissues involves processes that give rise to a variety of cell types within different tissues. Such cells within a particular tissue or organ are characterized by a unique
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Electrophoresis of brain proteins in polyacrylamide gel
Journal of Neurochemistry, 1964J, VOS, H J, VANDERHELM
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SDS Polyacrylamide Gel Electrophoresis of Proteins
1996Crosslinked polyacrylamide gels are formed from the polymerization of acrylamide monomer in the presence of smaller amounts of N,N'-methylene-bis-acrylamide (normally referred to as “bis-acrylamide”) (Fig. 1). Note that bis-acrylamide is essentially two acrylamide molecules linked by a methylene group and is used as a crosslinking agent.
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Gel electrophoresis of proteins
Journal of Chromatography B: Biomedical Sciences and Applications, 1987openaire +1 more source
Concentration of Dilute Protein for Gel Electrophoresis
Analytical Biochemistry, 1995D M, Sauvé, D T, Ho, M, Roberge
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