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Starch-gel Electrophoresis of Pig Serum Proteins [PDF]
Nature, 1963 MANY variations of the original technique of Smithies1 for starch-gel electrophoresis of proteins have been developed, notably the vertical gel2, the discontinuous buffer system3, and two-dimensional applications4,5. The latter method has been used by Ashton6 in examining pig serum proteins; it has one disadvantage in being time-consuming, requiring ...
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SDS Polyacrylamide Gel Electrophoresis of Proteins
1996Crosslinked polyacrylamide gels are formed from the polymerization of acrylamide monomer in the presence of smaller amounts of N,N'-methylene-bis-acrylamide (normally referred to as “bis-acrylamide”) (Fig. 1). Note that bis-acrylamide is essentially two acrylamide molecules linked by a methylene group and is used as a crosslinking agent.
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Studies of DNA‐protein interactions by gel electrophoresis
ELECTROPHORESIS, 1989AbstractThe use of gel electrophoresis in studies of nucleic acid‐protein (especially DNA‐protein) interactions has yielded much qualitative and quantitative information about a variety of such systems. The reduction in mobility of complexes relative to free DNA allows isolation and characterization of the complexes as well as determination of ...
Arnold Revzin, John A. Ceglarek
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Supramolecular Gel Electrophoresis of Acidic Native Proteins
Analytical Chemistry, 2014Amphiphilic tris-urea molecules self-assemble into a supramolecular hydrogel in tris(hydroxymethyl)aminomethane-glycine buffer. The supramolecular hydrogel is used as a matrix for the electrophoresis of acidic native proteins, in which proteins are separated based on their isoelectric points rather than their molecular weights.
Kanako Munenobu +3 more
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Gel electrophoresis in studies of protein conformation and folding
Analytical Biochemistry, 1984Electrophoresis through polyacrylamide gels is a useful method for distinguishing conformational states of proteins and analyzing the thermodynamic and kinetic properties of transitions between conformations. Although the relationship between protein conformation and electrophoretic mobility is quite complex, relative mobilities provide qualitative ...
David M. Goldenberg, Thomas E. Creighton
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Gel Electrophoresis of Proteins and Nucleic Acids
2017The electrophoresis technique describes migration of charged particles under the influence of an electric field. The rate of migration depends upon various factors like charge of the particle, applied electric field, and temperature and nature of the suspended medium. Gel electrophoresis is a technique in which the macromolecules like nucleic acids and
Suman Choudhary +2 more
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Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins
2003Since O'Farrell (1) introduced the improved technique for high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), it has become one of the most powerful tools for the separation and quantification of proteins from complex mixtures. The principal reason for this is that the method employs separation of denatured proteins according
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Polyacrylamide Gel Electrophoresis of Plant Proteins
1974Development is characterized by an ordered, controlled sequence of changes that occur within an organism at the molecular, cell, tissue and organ levels. Differentiation of cells and tissues involves processes that give rise to a variety of cell types within different tissues. Such cells within a particular tissue or organ are characterized by a unique
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One‐Dimensional Gel Electrophoresis of Proteins
Current Protocols in Immunology, 1991AbstractThis unit provides several methods for one‐dimensional gel electrophoresis of proteins. The basic protocol is the standard method used for gel electrophoresis under denaturing conditions. The first alternate protocol provides conditions used for nondenaturing gel electrophoresis.
Sean R. Gallagher, John A. S. Smith
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Artifacts and Common Errors in Protein Gel Electrophoresis
2018In spite of taking precautions, some common mistakes creep into well-planned gel electrophoresis experiments. This occurs commonly in relation to calculating the cross-linking factor of a gel, polymerization temperature and time for a polyacrylamide gel, inducing aggregates in samples for electrophoresis, titrating the running buffer in electrophoresis,
Biji T. Kurien +5 more
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