Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants. [PDF]
ABSTRACT Viral vectors can be useful tools for expressing recombinant proteins as well as delivering gene‐editing machinery. Despite their utility, the development and subsequent optimisation of these tools is often a difficult and tedious process.
Neubauer M +6 more
europepmc +2 more sources
Defender or accomplice? Dual roles of plant vesicle trafficking in restricting and enabling geminiviral systemic infection. [PDF]
Summary The vesicle trafficking system enables multidirectional cargo fluxes between endomembrane compartments. However, vesicle trafficking plays dual roles during pathogen infections. In plants, it mediates autophagic immune responses but can also be hijacked by pathogens to facilitate successful infections.
Cana-Quijada P +9 more
europepmc +2 more sources
Stabilisation of Tomato Yellow Leaf Curl China Virus Infectious Clones Through Micro-Homology Mediated End Joining. [PDF]
ABSTRACT Tomato yellow leaf curl China virus (TYLCCNV) is a major agricultural pathogen and primary model for circular single‐stranded DNA (cssDNA) virus studies. The infectious clones of TYLCCNV and other cssDNA viruses are usually constructed as two tandem copies of the small viral genomes in Agrobacterium‐mediated T‐DNA vectors.
Wang A +9 more
europepmc +2 more sources
REPercussions: how geminiviruses recruit host factors for replication
Circular single-stranded DNA viruses of the family Geminiviridae encode replication-associated protein (Rep), which is a multifunctional protein involved in virus DNA replication, transcription of virus genes, and suppression of host defense responses ...
Sara Shakir +8 more
doaj +1 more source
Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone. [PDF]
Viral diseases are important diseases on cash crops such as passion fruit, among which PaLCuGDV is one of the more severe in recent years. We constructed an infectious clone of PaLCuGDV for the first time and confirmed its infectivity through biological assays.
Xu S, Wu G, Yu Z, Yang C, Chen X.
europepmc +2 more sources
RepA Enhances Plant Resistance to Citrus Chlorotic Dwarf-Associated Virus by Transcriptionally Activating ClAOS. [PDF]
We revealed a novel role for CCDaV‐RepA as a nuclear transcriptional regulator and citrus utilise this function of RepA to activate ClAOS, which is a key gene that limits the rate of jasmonic acid (JA) synthesis. This enhances JA‐mediated defence response to CCDaV in lemon.
Zhao J, Zhang S, Dong J, Chen Y, Zhou Y.
europepmc +2 more sources
Virus-Inducible Activation of Hypersensitive Response Confers Broad-Spectrum Resistance to Diverse Viruses in Plants. [PDF]
Using a virus‐inducible promoter to drive the expression of the Avr4/Cf‐4 gene pair can trigger a hypersensitive response upon viral infection, which confers plants broad‐spectrum resistance to geminiviruses. ABSTRACT The hypersensitive response (HR) is a powerful plant defence mechanism that restricts pathogen spread through localised cell death ...
Pan RR +7 more
europepmc +2 more sources
Geminivirus particles, consisting of a pair of twinned isometric structures, have one of the most distinctive capsids in the virological world. Until recently, there was little information as to how these structures are generated.
Keith Saunders +3 more
doaj +1 more source
Geminivirus has a wide host range including cultivated plants and weeds. Infected weeds may play an important role in disease epidemic. Unfortunately, little is known about weeds species that may serve as alternative host for Geminivirus.
RIKA MELIANSYAH +2 more
doaj +1 more source
Background Soybean is one of the four major crops in China. The occurrence of viruses in soybean causes significant economic losses. Results In this study, the soybean leaves from stay-green plants showing crinkle were collected for metatranscriptomic ...
Qinglun Li +10 more
doaj +1 more source

