Results 351 to 360 of about 9,198,934 (404)
Some of the next articles are maybe not open access.
2008
Gene delivery by retroviruses is an easy and safe tool to stably over express a gene of interest and determine its role in a cell model. The gene of interest is cloned into the multiple cloning site of a retroviral vector that also contains a packaging signal and an antibiotic resistance marker for selection.
Valerie, Deregowski, Ernesto, Canalis
openaire +2 more sources
Gene delivery by retroviruses is an easy and safe tool to stably over express a gene of interest and determine its role in a cell model. The gene of interest is cloned into the multiple cloning site of a retroviral vector that also contains a packaging signal and an antibiotic resistance marker for selection.
Valerie, Deregowski, Ernesto, Canalis
openaire +2 more sources
2005
Polymeric gene delivery systems have been developed to overcome problems caused by viral carriers. They are low cytotoxic, have no size limit, are convenient in handling, of low cost and reproducible. A Terplex gene delivery system consisting of plasmid DNA, low density lipoprotein and hydropholized poly-L-lysine was designed and characterized.
openaire +3 more sources
Polymeric gene delivery systems have been developed to overcome problems caused by viral carriers. They are low cytotoxic, have no size limit, are convenient in handling, of low cost and reproducible. A Terplex gene delivery system consisting of plasmid DNA, low density lipoprotein and hydropholized poly-L-lysine was designed and characterized.
openaire +3 more sources
Expert Opinion on Drug Delivery, 2019
Introduction Despite the great therapeutic potential of gene therapy for treating critical diseases, the clinical application is limited by lack of safe and effective gene delivery vectors. Nonviral gene vectors have attracted tremendous attention due to
Cuiping Jiang +5 more
semanticscholar +1 more source
Introduction Despite the great therapeutic potential of gene therapy for treating critical diseases, the clinical application is limited by lack of safe and effective gene delivery vectors. Nonviral gene vectors have attracted tremendous attention due to
Cuiping Jiang +5 more
semanticscholar +1 more source
Sustainable cutaneous gene delivery
Nature Biotechnology, 1997Durable gene delivery to human skin is necessary for lasting correction of human genetic skin disease. Current cutaneous gene-delivery strategies, however, have achieved only transient gene expression, often only within a small percentage of tissue cells. The recent inability to sustain phenotypic correction of human genetic skin disease due to loss of
H, Deng, Q, Lin, P A, Khavari
openaire +2 more sources
Targetable Gene Delivery Vectors
2002Adenoviral vectors, which have targeting ligands for tumor cells on the capsid, no natural tropism, and carry a therapeutic payload should be constructed soon and tested in pre-clinical models. Nevertheless, there are still important considerations for the design and therapeutic use of targetable vectors.
P L, Hallenbeck, S C, Stevenson
openaire +2 more sources
Nanoparticle-Mediated Gene Delivery
2009Nonviral gene delivery has been gaining considerable attention recently. Although the efficacy of DNA transfection, which is a major concern, is low in nonviral vector-mediated gene transfer compared with viral ones, nonviral vectors are relatively easy to prepare, less immunogenic and oncogenic, and have no potential of virus recombination and no ...
Sha, Jin, John C, Leach, Kaiming, Ye
openaire +2 more sources
2008
Adenoviruses have a number advantages as gene delivery vectors, including ability to transduce a wide variety of non-dividing and dividing cells with high efficiency, relative ease of construction, and ability to be purified as high-titer viral stocks. These characteristics make adenoviruses particularly attractive for over-expressing specific genes in
Renny T, Franceschi, Chunxi, Ge
openaire +2 more sources
Adenoviruses have a number advantages as gene delivery vectors, including ability to transduce a wide variety of non-dividing and dividing cells with high efficiency, relative ease of construction, and ability to be purified as high-titer viral stocks. These characteristics make adenoviruses particularly attractive for over-expressing specific genes in
Renny T, Franceschi, Chunxi, Ge
openaire +2 more sources
Current Protocols in Human Genetics, 2001
AbstractThe delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or “knockout” mouse models.
Matthew L, Springer +2 more
openaire +2 more sources
AbstractThe delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or “knockout” mouse models.
Matthew L, Springer +2 more
openaire +2 more sources
Nanocarrier Stimuli‐Activated Gene Delivery
Small, 2006Udgivelsesdato: 2007 ...
Howard, Kenneth A +6 more
openaire +3 more sources
Polycations for Gene Delivery: Dilemmas and Solutions.
Bioconjugate chemistry, 2018Gene therapy has been a promising strategy for treating numerous gene-associated human diseases by altering specific gene expressions in pathological cells.
Jie Chen +4 more
semanticscholar +1 more source