Results 241 to 250 of about 13,637,089 (296)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Relationships between gene expression variability, expression levels, and Protein–Protein interactions in mouse and yeast

PLoS ONE
Peter M. Palenchar
doaj  

The evolution of gene expression levels in mammalian organs

Nature, 2011
Changes in gene expression are thought to underlie many of the phenotypic differences between species. However, large-scale analyses of gene expression evolution were until recently prevented by technological limitations. Here we report the sequencing of polyadenylated RNA from six organs across ten species that represent all major mammalian lineages ...
Gabor Csárdi, Henrik Kaessmann, Martin Kircher   +2 more
exaly   +7 more sources

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mRNAs, proteins and the emerging principles of gene expression control

Nature Reviews Genetics, 2020
Christopher Buccitelli, Matthias Selbach   +1 more
exaly   +2 more sources

Transient Gene Expression Levels from Multigene Expression Vectors

Biotechnology Progress, 2007
AbstractMultigene expression vectors are commonly utilized whereby the (over)expression of two or more genes is desired simultaneously and often at supposedly equivalent levels. We have characterized dual‐gene and pEE14.4 RlucMFluc expression plasmids in which the second hCMV‐MIE promoter is replaced with a c‐myc IRES to enable one‐step coordinated ...
Underhill, MF, Smales, CM, Naylor, LH, Birch, JR, James, DC   +4 more
openaire   +5 more sources

Base composition and expression level of human genes

Gene, 2004
It is well known that the gene distribution is non-uniform in the human genome, reaching the highest concentration in the GC-rich isochores. Also the amino acid frequencies, and the hydrophobicity, of the corresponding encoded proteins are affected by the high GC level of the genes localized in the GC-rich isochores.
Stilianos, Arhondakis, Fabio, Auletta, Giuseppe, Torelli, Giuseppe, D'Onofrio   +3 more
openaire   +2 more sources

On/off regulation of gene expression at the level of splicing

Trends in Genetics, 1988
Abstract The past two years have seen the discovery of three independent cases in which expression of a eukaryotic protein gene product is turned on and off by controlling splicing events necessary to produce the corresponding mRNA. Various considerations suggest that such on/off regulation at the level of splicing may be unexpectedly common.
P M, Bingham, T B, Chou, I, Mims, Z, Zachar   +3 more
openaire   +2 more sources

Gene-expression analysis at the single-cell level

Trends in Pharmacological Sciences, 2000
The manner in which a cell responds to and influences its environment is ultimately determined by the genes that it expresses. To fully understand and manipulate cellular function, identification of these expressed genes is essential. Techniques such as RT-PCR enable examination of gene expression at the tissue level.
A K, Dixon, P J, Richardson, R D, Pinnock, K, Lee   +3 more
openaire   +2 more sources

On DNA Computers Controlling Gene Expression Levels

Proceedings of the 44th IEEE Conference on Decision and Control, 2006
The area of bio-molecular computing has recently witnessed a major paradigm shift. Rather then being used only as simple calculating units capable of solving hard combinatorial or numerical problems, DNA computers are increasingly becoming more tailored to operate like intelligent ent biological machines with unprecedented potentials.
Olgica Milenkovic, Navin Kashyap, Bane Vasic   +2 more
openaire   +1 more source