Results 121 to 130 of about 946,920 (339)
bioRxiv, 2018 Here we present Perturb-ATAC, a method which combines multiplexed CRISPR interference or knockout with genome-wide chromatin accessibility profiling in single cells, based on the simultaneous detection of CRISPR guide RNAs and open chromatin sites by ...
Adam J. Rubin +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Modeling of Multiple Valued Gene Regulatory Networks [PDF]
, 2007 In silico modeling of Gene Regulatory Networks has gained a lot of attention recently as it gives a very powerful tool to experimental biologists to gather the knowledge gained from different biological experiments and understand the dynamics of the ...
De Micheli, Giovanni +7 more
core +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Gene regulatory networks and cell lineages that underlie the formation of skeletal muscle
Proceedings of the National Academy of Sciences of the United States of America, 2017 Skeletal muscle in vertebrates is formed by two major routes, as illustrated by the mouse embryo. Somites give rise to myogenic progenitors that form all of the muscles of the trunk and limbs. The behavior of these cells and their entry into the myogenic
M. Buckingham
semanticscholar +1 more source
Plant gene regulatory networks : methods and protocols
, 2023 Plant development and environmental responses require the coordinated, orchestrated, and often cell-type-specific activities of many gene products. The complex regulatory relationships between genes and gene products are abstractly described as gene ...
Vandepoele, Klaas, Kaufmann, Kerstin
core +1 more source
FEBS Letters, EarlyView.Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani +3 more
wiley +1 more source
Reconstructing Dynamic Gene Regulatory Networks Using f-Divergence from Time-Series scRNA-Seq Data
Current Issues in Molecular BiologyInferring time-varying gene regulatory networks from time-series single-cell RNA sequencing (scRNA-seq) data remains a challenging task. The existing methods have notable limitations as most are either designed for reconstructing time-varying networks ...
Yunge Wang +5 more
doaj +1 more source
Modelling gene regulatory networks using computational intelligence techniques
, 2010 This chapter presents modelling gene regulatory networks (GRNs) using probabilistic causal model and the guided genetic algorithm. The problem of modelling is explained from both a biological and computational perspective.
Chetty, Madhu, Ram, Ramesh
core
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source