Results 31 to 40 of about 4,429,101 (170)

Serological and molecular biological analysis of RhD--: a case report

Zhongguo shuxue zazhi, 2023
Objective To study the blood group serology and molecular biology of patients with RhD--, so as to guide clinical blood use. Methods The EDTA-K2 anticoagulant blood of the patient was detected for Rh antigens and antibodies. Meanwhile, DNA was extracted,
Bo SHUI, Zhibing HOU, Li YAO, Wei YAN, Jiwu HE   +4 more
doaj   +1 more source

Methods, Challenges and Potentials of Single Cell RNA-seq [PDF]

, 2012
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene
Daniel Hebenstreit, Hebenstreit, Daniel
core   +1 more source

Improved annotation of 3' untranslated regions and complex loci by combination of strand-specific direct RNA sequencing, RNA-seq and ESTs [PDF]

, 2014
The reference annotations made for a genome sequence provide the framework for all subsequent analyses of the genome. Correct and complete annotation in addition to the underlying genomic sequence is particularly important when interpreting the results ...
Junfang Song, Geoffrey J Barton, Schurch Nicholas J., Brown, Sara J., Gordon G Simpson, Nicholas J Schurch, McLean, W. H. Irwin, Song Junfang, Simpson Gordon G., Sherstnev, Alexander, Brown Sara J., Alexander Sherstnev, Song, Junfang, McLean W. H. Irwin, Duc, Celine, Kate G Storey, Storey, Kate G., Schurch, Nicholas J., Cole Christian, Sherstnev Alexander, Simpson, Gordon G., Céline Duc, W H Irwin McLean, Cole, Christian; id_orcid, Christian Cole, Barton Geoffrey J., Sara J Brown, Duc Céline, Barton, Geoffrey J.; id_orcid, Storey Kate G.   +29 more
core   +1 more source

TMEtyper: A computational method for tumor microenvironment subtyping with applications in immunotherapy

Computational and Structural Biotechnology Journal
Background: The heterogeneity of the tumor microenvironment (TME) is a critical determinant of outcomes in immune checkpoint blockade (ICB) therapy.
Yaru Miao, Tong Zhou, Yan Li, Jie Yao, Yanghui Bi, Ruiping Zhang   +5 more
doaj   +1 more source

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +2 more sources

A new RT-PCR assay for the revealing of Newcastle disease viruses by designing a pair of universal primers [PDF]

Iraqi Journal of Veterinary Sciences
Newcastle disease viruses (NDVs) possess a single-stranded, non-segmented RNA and are classified into different strains based on their level of pathogenicity.
Firas T. Al-Mubarak, Afnan G. Yaqoub, Manar M. Alnassar   +2 more
doaj   +1 more source

cnvCapSeq: detecting copy number variation in long-range targeted resequencing data. [PDF]

, 2014
Targeted resequencing technologies have allowed for efficient and cost-effective detection of genomic variants in specific regions of interest. Although capture sequencing has been primarily used for investigating single nucleotide variants and indels ...
Bellos, Evangelos; https://orcid.org/, Bellos, Evangelos, Kumar, Vikrant, Cheng, C-Y, Cheng, CY, Wong, TYI, Davila, S, Bellos, E, Phua, ZYA, Maggi, Jordi; https://orcid.org/, Lin, C, Phua, Zai Yang, Coin, Lachlan J M, Coin, LJM, Hibberd, ML, Hibberd, Martin L., Wong, TY, Cheng, Ching-Yu, Gemmy Cheung, CM, Cheung, CMIG, Coin, Lachlan JM, Yang Phua, Z, Lin, Clarabelle, Maggi, Jordi, Kumar, V, Cheng, Ching Yu, Maggi, J, Hibberd, Martin L, Coin, Lachlan J.M., Cheung, Chui Ming Gemmy, Davila, Sonia, Wong, Tien Yin   +31 more
core   +1 more source

Sequencing BGI: the evolution of expertise and research organisation in the world’s leading gene sequencing facility

New Genetics and Society, 2021
The increasing importance of computational techniques in post-genomic life science research calls for new forms and combinations of expertise that cut across established disciplinary boundaries between computing and biology.
Kai Wang, Xiaobai Shen, Robin Williams
doaj   +1 more source

Quantifying single nucleotide variant detection sensitivity in exome sequencing [PDF]

, 2013
BACKGROUND: The targeted capture and sequencing of genomic regions has rapidlydemonstrated its utility in genetic studies. Inherent in this technology isconsiderable heterogeneity of target coverage and this is expected tosystematically impact our ...
Taylor, Martin S.; id_orcid, Andrew P Jackson, Alison M Meynert, Jackson, Andrew, Bicknell, Louise, Martin S Taylor, Matthew E Hurles, Meynert, Alison, Louise S Bicknell   +8 more
core   +1 more source

Mucosal immunity in cancer metastasis: roles, mechanisms, and therapeutic implications

Frontiers in Immunology
Previous studies have shown that the mucosal immune system plays a crucial role in regulating immune tolerance, maintaining the integrity of the mucosal barrier, and facilitating immune communication between organs.
Yaru Miao, Dongdong Cui, Yanghui Bi, Ruiping Zhang   +3 more
doaj   +1 more source