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Genetic Manipulation for Improvement of Microbial Biocontrol Agents

1999
During the last few years genetic modification of microorganisms to improve their biological control of plant pathogens has created some very interesting results, which indicates that molecular biology can be used not only to understand the mechanism behind biological control, but also to improve biocontrol ability.
Sonja Sletner Klemsdal, Arne Tronsmo
openaire   +1 more source

Genetic Improvement of Entomopathogenic Nematodes for Insect Biocontrol

2002
Entomopathogenic nematodes (families Heterorhabditidae and Steinernematidae) are the most important biological-control agents for soil-dwelling insect pests (Gaugler and Kaya, 1990). Several species of Heterorhabditids and Steinernematids are produced commercially and used as biotic insecticides.
Christopher W. Brey, Sarwar Hashmi
openaire   +1 more source

Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper

Journal of Basic Microbiology, 2019
Trichoderma species are recognized as biocontrol agents with great potential in inhibiting fungal pathogens that cause significant crop losses. In this study, 15 Trichoderma isolates were collected from various Egyptian locations.
O. Hewedy, K. Abdel-Lateif, R. Bakr
semanticscholar   +1 more source

Molecular Genetics of the Biocontrol Agents Trichoderma spp

1991
ABSTRACT We have developed conditions for an efficient method of genetic transformation for the biocontrol agents Trichoderma spp, using polyethylene glycol (PEG) and CaCl 2 or high-voltage electroporation. Transformation was obtained with the plasmid pAN7–1 carrying a bacterial hygromycin resistance gene as a selectable marker under the control ...
G.H. GOLDMAN   +3 more
openaire   +1 more source

Genetic Improvement of Biocontrol Agents for Sustainable Pest Management

2014
Genetic improvement involves directed purposeful genetic alterations to enhance the efficacy of natural enemies for biological control. This may be achieved by conventional approaches as well as through recombinant DNA techniques. The conventional methods include strain selection, serial passage through hosts, mutation, conjugation, transduction ...
Ramesh Arora, P. S. Shera
openaire   +1 more source

Investigating the potential of X shredding for mouse genetic biocontrol

2023
AbstractCRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims to bias offspring towards males, resulting in a gradual and unsustainable decline of females.
Mark. D. Bunting   +8 more
openaire   +1 more source

Biocontrol potential and genetic diversity of Metarhizium anisopliae lineage in agricultural habitats

Journal of Applied Microbiology, 2019
To assess phylogenetic and genotypic diversity of Metarhizium anisopliae lineage within diverse agroecosystems in the Karnataka State of India and to compare their chitinase activity and pathogenicity against insect pest of field crops subterranean ...
J. Francis
semanticscholar   +1 more source

Biocontrol ability of fluorescent pseudomonads genetically dissected: importance of positive feedback regulation

Current Opinion in Biotechnology, 2000
Root diseases caused by fungal pathogens can be suppressed by certain rhizobacteria that effectively colonize the roots and produce extracellular antifungal compounds. To be effective, biocontrol bacteria need to be present at sufficiently high cell densities.
D, Haas, C, Blumer, C, Keel
openaire   +2 more sources

Genetically Engineered Microbial Agents for Biocontrol of Plant Fungal Diseases

2005
The objectives of the project were: a) to construct the site-specific integrative expression cassettes carrying: (i) the chiA gene for a 58-kDa endochitinase, (ii) the pyrrolnitrin biosynthesis operon, and (iii) the acdS gene encoding ACC deaminase; b) to employ these constructs to engineer stable recombinant strains with an expanded repertoire of ...
Linda Thomashow   +4 more
openaire   +1 more source

Re-evaluation of biosafety questions on genetically modified biocontrol bacteria

European Journal of Plant Pathology, 2018
Microorganisms have the potential to promote plant health and can be used to increase agricultural production that depends less on chemical control. The implementation of EU Directive 2009/128/EC, also called the Sustainable Use Directive, has led to a renewed interest in microbial biocontrol of plant diseases.
openaire   +2 more sources

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