Results 101 to 110 of about 1,127,408 (302)

Genetic Engineering in Bacteria, Fungi, and Oomycetes, Taking Advantage of CRISPR

DNA
Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances in genetic engineering techniques for bacteria, fungi, and oomycetes, with a
Piao Yang, Abraham Condrich, Ling Lu, Sean Scranton, Camina Hebner, Mohsen Sheykhhasan, Muhammad Azam Ali   +6 more
doaj   +1 more source

Plasticity of the HEK-293 cells, related to the culture media, as platform to produce a subunit vaccine against classical swine fever virus

AMB Express, 2019
Classical swine fever (CSF) is a contagious disease that causes a high mortality to domestic and wild pigs. Its causative agent is an enveloped Pestivirus named Classical Swine Fever Virus (CSFV).
Elianet Lorenzo, Lidice Méndez, Elsa Rodríguez, Nemecio Gonzalez, Gleysin Cabrera, Carlos Pérez, Rafael Pimentel, Yusmel Sordo, Maria P. Molto, Talia Sardina, Alina Rodríguez-Mallon, Mario P. Estrada   +11 more
doaj   +1 more source

Defining the Agricultural Biotechnology Policy of the Philippines [PDF]

Agricultural biotechnology offers a whole new range of possibilities to solving the problems that impinge on the productivity of the agriculture sector and its ability to feed the growing human population.
Halos, Saturnina C.
core  

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Genetic Insights Into Type 2 Diabetes Mellitus Susceptibility: A Case-Control Study of the ADIPOQ rs1501299 Polymorphism in the Population of Noakhali Region of Bangladesh

Genetics Research
Type 2 diabetes mellitus (T2DM) is a global health concern, particularly prevalent in low to middle-income countries like Bangladesh. This case-control study aims to explore the correlation between the ADIPOQ rs1501299 polymorphism and susceptibility to ...
Md. Anamul Haque, Md. Sad Salabi Sawrav, Shipan Das Gupta, Shuvo Chandra Das, Dhirendra Nath Barman, Mohammed Mafizul Islam, Md. Murad Hossain   +6 more
doaj   +1 more source

Feature engineering through two-level genetic algorithm

Machine Learning with Applications
Deep learning models are widely used for their high predictive performance, but often lack interpretability. Traditional machine learning methods, such as logistic regression and ensemble models, offer greater interpretability but typically have lower ...
Aditi Gulati, Armin Felahatpisheh, Camilo E. Valderrama   +2 more
doaj   +1 more source

Regulating Human Gene Therapy [PDF]

, 1985
Scientific developments have moved the public debate on genetic engineering to the issue of human gene therapy. Because so many important societal values must be weighed in deciding which, if any, of the first protocols for human gene therapy should be ...
Areen, Judith C.
core   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source