Results 31 to 40 of about 547,025 (332)

An Optimized and Efficient CRISPR/Cas9 System for the Endophytic Fungus Pestalotiopsis fici

open access: yesJournal of Fungi, 2021
Endophytic fungi are emerging as attractive producers of natural products with diverse bioactivities and novel structures. However, difficulties in the genetic manipulation of endophytic fungi limit the search of novel secondary metabolites.
Xinran Xu, Runye Huang, Wen-Bing Yin
doaj   +1 more source

Prospects for transgenesis in the chick [PDF]

open access: yes, 2004
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick
Bosselman   +52 more
core   +1 more source

Molecular Responses to High Hydrostatic Pressure in Eukaryotes: Genetic Insights from Studies on Saccharomyces cerevisiae

open access: yesBiology, 2021
High hydrostatic pressure is common mechanical stress in nature and is also experienced by the human body. Organisms in the Challenger Deep of the Mariana Trench are habitually exposed to pressures up to 110 MPa.
Fumiyoshi Abe
doaj   +1 more source

Identification of 5-Fluoro-5-Deoxy-Ribulose as a Shunt Fluorometabolite in Streptomyces sp. MA37

open access: yesBiomolecules, 2020
A fluorometabolite, 5-fluoro-5-deoxy-D-ribulose (5-FDRul), from the culture broth of the soil bacterium Streptomyces sp. MA37, was identified through a combination of genetic manipulation, chemo-enzymatic synthesis and NMR comparison.
Linrui Wu   +3 more
doaj   +1 more source

Near-Complete Genome Assembly of Alternaria brassicae—A Necrotrophic Pathogen of Brassica Crops

open access: yesMolecular Plant-Microbe Interactions, 2019
Alternaria brassicae, a necrotrophic fungal pathogen, causes Alternaria blight, an important disease of brassica crops. Although many Alternaria spp. have been sequenced, no genome information is available for A. brassicae, a monotypic lineage within the
Sivasubramanian Rajarammohan   +2 more
doaj   +1 more source

Laser microdissection as a new approach to prefertilization genetic diagnosis [PDF]

open access: yes, 2000
The genetic status of oocytes can be determined by polar body (PB) analysis. Following PB extraction, a genetic evaluation is performed. As each PB contains the complementary genetic material of the oocyte, PB analysis reveals information about its ...
Buchholz, Tina   +2 more
core   +1 more source

Genetiese manipulasie as selektiewe voortplanting – ’n voorlopige teologies- etiese beoordeling

open access: yesIn die Skriflig, 2006
Genetic manipulation as selective reproduction – a preliminary theological-ethical assessment Genetic manipulation – the technology in which genes are placed in embryos and gametes of animals – with the purpose of creating certain intended genetic ...
A. L. Rheeder, J. M. Vorster
doaj   +1 more source

In vivo genetic manipulation of inner ear connexin expression by bovine adeno-Associated viral vectors [PDF]

open access: yes, 2017
We have previously shown that in vitro transduction with bovine adeno-associated viral (BAAV) vectors restores connexin expression and rescues gap junction coupling in cochlear organotypic cultures from connexin-deficient mice that are models DFNB1 ...
Campioni, Matteo   +7 more
core   +1 more source

CRISPR/Cas9 Systems for the Development of Saccharomyces cerevisiae Cell Factories

open access: yesFrontiers in Bioengineering and Biotechnology, 2020
Synthetic yeast cell factories provide a remarkable solution for the sustainable supply of a range of products, ranging from large-scale industrial chemicals to high-value pharmaceutical compounds. Synthetic biology is a field in which metabolic pathways
Jie Meng, Yue Qiu, Shuobo Shi
doaj   +1 more source

Enhanced flight performance by genetic manipulation of wing shape in Drosophila [PDF]

open access: yes, 2016
Insect wing shapes are remarkably diverse and the combination of shape and kinematics determines both aerial capabilities and power requirements. However, the contribution of any specific morphological feature to performance is not known.
A Censi   +40 more
core   +2 more sources

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