Results 91 to 100 of about 2,453,394 (283)

Function of bacteriophage orf recombinasesin genetic exchange [PDF]

, 2006
Recombination events in bacteriophages frequently occur by illegitimate exchange at short tracts of sequence homology, enabling these viruses to acquire novel genes and serve as vehicles for horizontal gene transfer.
Reed, Patricia
core  

Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections? [PDF]

, 2011
Background: The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans.
Boontanom, P., Mungthin, M., Thompson, R.C.A., Tan-ariya, P., Siripattanapipong, S., Saksirisampant, W., Mathirut Mungthin, Peerapan Tan-ariya, RC Andrew Thompson, Leelayoova, S., Suradej Siripattanapipong, Wilai Saksirisampant, Parima Boontanom, Saovanee Leelayoova   +13 more
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Genetic Engineering by DNA Recombineering

Current Protocols in Chemical Biology, 2019
AbstractRecombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introduced as 5′ primer overhangs during the PCR reaction.
Papa, Louis John, Shoulders, Matthew D.
openaire   +5 more sources

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Diversity and recombination in Wolbachia and Cardinium from Bryobia spider mites [PDF]

, 2012
Background Wolbachia and Cardinium are endosymbiotic bacteria infecting many arthropods and manipulating host reproduction. Although these bacteria are maternally transmitted, incongruencies between phylogenies of host and parasite suggest an additional ...
Vera I D Ros, Edward J Feil, Johannes A J Breeuwer, Breeuwer, J.A.J., Fleming, V.M., Feil, E.J., Ros, V.I.D., Vicki M Fleming   +7 more
core   +2 more sources

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Isolation and characterization of "Arabidopsis" mutants with altered homologous recombination levels : a new function for an INO80 SWI/SNF ATPase [PDF]

, 2006
Homologous recombination (HR) in eukaryotic organisms serves a dual role in providing genetic flexibility by creating novel sequence assortments upon meiosis and in maintaining genome integrity through DNA repair in somatic tissues.
Fritsch, Olivier
core   +1 more source

Optimization of the Production of Rubber Compounds Using Mathematical Models

Advanced Engineering Materials, EarlyView.
Rubber compounds were mixed in a batch internal mixer, and symbolic regression was used to derive mathematical models linking recipe and process parameters to ram path, torque, and mixing quality (incorporation, dispersion, distribution). Subsequent optimization with evolutionary algorithms identified operating conditions that reduce specific energy ...
Anke Bardehle, Andreas Limper, Ulrich Giese, Carlos Lucke, Mario Beiner, Christoph Gögelein, Lisa Leuchtenberger‐Engel, Christian Hopmann   +7 more
wiley   +1 more source

The Moran model of population genetics : case studies with recombination and selection [PDF]

, 2012
Hustedt T. The Moran model of population genetics : case studies with recombination and selection. Bielefeld: Universität Bielefeld; 2012.The Moran model is a widespread model for a finite population in the field of population genetics.
Hustedt, Thiemo
core