Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Factors influencing trustworthiness and perceived biases of medical information and genetic testing for Black and White Americans. [PDF]
Smith CL +7 more
europepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Biallelic TMEM126B Variants as a Novel Cause of Kidney Failure-Implications for Mitochondrial Genetic Testing in Nephrology. [PDF]
Sentell ZT +7 more
europepmc +1 more source
Live birth rates with and without preimplantation genetic testing: a single-center retrospective study. [PDF]
Wang Y +5 more
europepmc +1 more source
A case of concurrent Alport syndrome and Nail-patella syndrome posing diagnostic challenge without genetic testing. [PDF]
Fung WW +5 more
europepmc +1 more source
Ethical, legal, and policy dimensions and contentions for reanalysis and reinterpretation of clinical genetic testing results. [PDF]
Sentell ZT, Zawati MH.
europepmc +1 more source
Genetic Testing Among Medicaid-Insured Children With Autism and Intellectual Disability.
Brown TR +7 more
europepmc +1 more source
Systematic Review: The Psychosocial Impacts of Autism-Related Genetic Testing. [PDF]
Wills BC +5 more
europepmc +1 more source

