Results 301 to 310 of about 1,004,687 (332)
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Genetic transformation of fungi
The International Journal of Developmental Biology, 2017Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely ...
Chunmei Chen +7 more
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2011
Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Tubercle, 1989
Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
Barry R. Bloom +2 more
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Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
Barry R. Bloom +2 more
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Genetic transformation of mycobacteria [PDF]
Trends in Microbiology, 1993 Recent advances in methods for introducing DNA into both fast- and slow-growing species of mycobacteria have stimulated the construction of a variety of cloning vectors and the development of a versatile genetic system. These tools will facilitate studies of mycobacterial pathogenicity, antibiotic action and drug resistance, and lead to more effective ...
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Genetic Transformation of Switchgrass
2009Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used
Zeng-Yu Wang, Yajun Xi, Yaxin Ge
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The Genetics of Transformation
1961Publisher Summary This chapter discusses the chemical nature of the transforming agent and mechanism of recombination occurring in transformation. The investigation of pneumococcal transformations was concerned largely with the chemical identification of the substance in the donor extract that was capable of inducing genetic transformations.
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Genetics of drug transformation
Clinical Biochemistry, 1986Biotransformations of drugs are controlled or strongly affected by genetic factors. During the past few years several genetic deficiencies of drug-metabolizing reactions catalyzed by members of the family of cytochrome P-450 were observed. Choice of the appropriate drug to study and attention to urinary metabolites have been the essential ingredients ...
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Genetic Transformation in Citrus [PDF]
, 2000 More Citrus is produced on a world-wide basis than any other fruit crop. The genus includes a number of species grown for fruit and/or juice products, including sweet oranges (C. sinensis [L.] Osbeck), grapefruit (C. paradisi Macf.), mandarins (C. reticulata Blanco), lemons (C. limon [L.] Burm. f.), and limes (C.
Fatma Kaplan +3 more
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Genetic Transformation of Pineapple
2018Transgenic pineapple plants have been produced for the purpose of improving various economically important traits. The efforts have focused on traits such as natural flowering control, herbicide tolerance for weed control, disease and nematode control, and fruit quality traits.
Ming-Li Wang, Robert E. Paull
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Genetic Transformation of Rice
Critical Reviews in Plant Sciences, 1994Abstract Rice was the first major monocot crop species to be transformed and regenerated. Initially, rice transformation was limited to japonica cultivars. Subsequently, a number of indica and javanica cultivars have also been transformed and regenerated into fertile transgenic plants.
Nicola M. Ayres, William D. Park
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