Results 311 to 320 of about 5,465,577 (394)

An Improved and Simplified Agrobacterium-Mediated Genetic Transformation Protocol for Solanum nigrum with a Shorter Growth Time. [PDF]

Plants (Basel)
Li Q, Wang X, Teng C, He X, Fu X, Peng W, Fan Y, Lyu S.   +7 more
europepmc   +1 more source

Identifying prognostic targets in metastatic prostate cancer beyond AR

FEBS Open Bio, EarlyView.
Genome‐wide functional screens combined with a large gene expression database and clinical outcomes can identify new therapeutic vulnerabilities in prostate cancer. Eight potentially druggable targets demonstrated strong dependency in cell lines, were associated with worse prognosis clinically, and showed evidence of protein expression in prostate ...
Emily Feng, Eric Feng, Tracy Berg, Isabella S. Nguyen, Lilac G. Nguyen, William Chen, Meng Zhang, David Quigley, Marina Sharifi, Haolong Li, Ilsa Coleman, Peter S. Nelson, Martin Sjöström, Shuang G. Zhao   +13 more
wiley   +1 more source

Genetic transformation of the frog-killing chytrid fungus Batrachochytrium dendrobatidis. [PDF]

Proc Natl Acad Sci U S A
Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK.   +4 more
europepmc   +1 more source

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

Construction and optimization of a genetic transformation system for efficient expression of human insulin-GFP fusion gene in flax. [PDF]

Bioresour Bioprocess
Zhao W, Zhang R, Zhou L, Zhang Z, Du F, Wu R, Kong J, An S.   +7 more
europepmc   +1 more source

Current trends in single‐cell RNA sequencing applications in diabetes mellitus

FEBS Open Bio, EarlyView.
Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian, Khodakaram Jahanbin, Shekoofeh Nikooei, Marzieh Rostaminejad, Arezoo Rahimi, Pedram Abdizadeh, Behnam Alipoor   +6 more
wiley   +1 more source

Genetic Transformation of Triticum dicoccum and Triticum aestivum with Genes of Jasmonate Biosynthesis Pathway Affects Growth and Productivity Characteristics. [PDF]

Plants (Basel)
Miroshnichenko DN, Pigolev AV, Pushin AS, Alekseeva VV, Degtyaryova VI, Degtyaryov EA, Pronina IV, Frolov A, Dolgov SV, Savchenko TV.   +9 more
europepmc   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

A method of genetic transformation and gene editing of succulents without tissue culture. [PDF]

Plant Biotechnol J
Lu J, Li S, Deng S, Wang M, Wu Y, Li M, Dong J, Lu S, Su C, Li G, Lang Z, Zhu JK.   +11 more
europepmc   +1 more source

Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation. [PDF]

Bio Protoc
Siamalube B, Ehinmitan E, Ngotho M, Onguso J, Runo S.   +4 more
europepmc   +1 more source